This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garo, E.
Right arrow Articles by James, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garo, E.
Right arrow Articles by James, G. A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, May 2007, p. 1813-1817, Vol. 51, No. 5
0066-4804/07/$08.00+0     doi:10.1128/AAC.01037-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Asiatic Acid and Corosolic Acid Enhance the Susceptibility of Pseudomonas aeruginosa Biofilms to Tobramycin{triangledown}

Eliane Garo,1* Gary R. Eldridge,2* Matt G. Goering,2 Elinor DeLancey Pulcini,3 Martin A. Hamilton,3 John W. Costerton,3 and Garth A. James3

Sequoia Sciences Inc., 11199 Sorrento Valley Road, Suite H, San Diego, California 92121,1 Sequoia Sciences Inc., 1912 Innerbelt Business Center Drive, Saint Louis, Missouri 63114,2 Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717-39803

Received 18 August 2006/ Returned for modification 27 December 2006/ Accepted 2 March 2007

Asiatic acid and corosolic acid are two natural products identified as biofilm inhibitors in a biofilm inhibition assay. We evaluated the activities of these two compounds on Pseudomonas aeruginosa biofilms grown in rotating disk reactors (RDRs) in combination with tobramycin and ciprofloxacin. To determine the ruggedness of our systems, the antibiotic susceptibilities of these biofilms were assessed with tobramycin and ciprofloxacin. The biofilm bacteria produced in the RDR were shown to display remarkable tolerance to 10 µg/ml of ciprofloxacin, thus mimicking the tolerance observed in recalcitrant bacterial infections. These studies further demonstrate that a nonmucoid strain of P. aeruginosa can form a biofilm that tolerates ciprofloxacin at clinically relevant concentrations. Neither asiatic acid nor corosolic acid reduced the viable cell density of P. aeruginosa biofilms. However, both compounds increased the susceptibility of biofilm bacteria to subsequent treatment with tobramycin, suggesting asiatic acid and corosolic acid to be compounds that potentiate the activity of antibiotics. A similar statistical interaction was observed between ciprofloxacin and subsequent treatment with tobramycin.

* Corresponding author. Mailing address: Sequoia Sciences Inc., 1912 Innerbelt Business Center Drive, Saint Louis, MO 63114. Phone: (314) 373-5181. Fax: (314) 373-5186. E-mail for Eliane Garo: egaro{at}sequoiasciences.com. E-mail for Gary R. Eldridge: geldridge{at}sequoiasciences.com

{triangledown} Published ahead of print on 12 March 2007.

Antimicrobial Agents and Chemotherapy, May 2007, p. 1813-1817, Vol. 51, No. 5
0066-4804/07/$08.00+0     doi:10.1128/AAC.01037-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»