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Antimicrobial Agents and Chemotherapy, July 2007, p. 2464-2469, Vol. 51, No. 7
0066-4804/07/$08.00+0     doi:10.1128/AAC.00143-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Transferable Resistance to Aminoglycosides by Methylation of G1405 in 16S rRNA and to Hydrophilic Fluoroquinolones by QepA-Mediated Efflux in Escherichia coli{triangledown}

Bruno Périchon, Patrice Courvalin, and Marc Galimand*

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France

Received 31 January 2007/ Returned for modification 24 March 2007/ Accepted 24 April 2007

Plasmid pIP1206 was detected in Escherichia coli strain 1540 during the screening of clinical isolates of Enterobacteriaceae for high-level resistance to aminoglycosides. The sequence of this IncFI conjugative plasmid of ca. 100 kb was partially determined. pIP1206 carried the rmtB gene for a ribosome methyltransferase that was shown to modify the N7 position of nucleotide G1405, located in the A site of 16S rRNA. It also contained the qepA (quinolone efflux pump) gene that encodes a 14-transmembrane-segment putative efflux pump belonging to the major facilitator superfamily of proton-dependent transporters. Disruption of membrane proton potential by the efflux pump inhibitor carbonyl cyanide m-chlorophenylhydrazone in a transconjugant harboring the qepA gene resulted in elevation of norfloxacin accumulation. The transporter conferred resistance to the hydrophilic quinolones norfloxacin and ciprofloxacin.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33-1-45-68-83-18. Fax: 33-1-45-68-83-19. E-mail: galimand{at}pasteur.fr

{triangledown} Published ahead of print on 30 April 2007.

Antimicrobial Agents and Chemotherapy, July 2007, p. 2464-2469, Vol. 51, No. 7
0066-4804/07/$08.00+0     doi:10.1128/AAC.00143-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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