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Antimicrobial Agents and Chemotherapy, August 2007, p. 2774-2783, Vol. 51, No. 8
0066-4804/07/$08.00+0     doi:10.1128/AAC.01127-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Promoter and Transcription Analysis of Penicillin-Binding Protein Genes in Streptococcus gordonii

Marisa Haenni, Philippe Moreillon,^* and Vladimir Lazarevic

Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland

Received 7 September 2006/ Returned for modification 17 December 2006/ Accepted 7 May 2007

An optimally cross-linked peptidoglycan requires both transglycosylation and transpeptidation, provided by class A and class B penicillin-binding proteins (PBPs). Streptococcus gordonii possesses three class A PBPs (PBPs 1A, 1B, and 2A) and two class B PBPs (PBPs 2B and 2X) that are important for penicillin resistance. High-level resistance (MIC, 2 µg/ml) requires mutations in class B PBPs. However, although unmutated, class A PBPs are critical to facilitate resistance development (M. Haenni and P. Moreillon, Antimicrob. Agents Chemother. 50:4053-4061, 2006). Thus, their overexpression might be important to sustain the drug. Here, we determined the promoter regions of the S. gordonii PBPs and compared them to those of other streptococci. The extended –10 box was highly conserved and complied with a ^A-type promoter consensus sequence. In contrast, the –35 box was poorly conserved, leaving the possibility of differential PBP regulation. Gene expression in a penicillin-susceptible parent (MIC, 0.008 µg/ml) and a high-level-resistant mutant (MIC, 2 µg/ml) was monitored using luciferase fusions. In the absence of penicillin, all PBPs were constitutively expressed, but their expression was globally increased (1.5 to 2 times) in the resistant mutant. In the presence of penicillin, class A PBPs were specifically overexpressed both in the parent (PBP 2A) and in the resistant mutant (PBPs 1A and 2A). By increasing transglycosylation, class A PBPs could promote peptidoglycan stability when transpeptidase is inhibited by penicillin. Since penicillin-related induction of class A PBPs occurred in both susceptible and resistant cells, such a mutation-independent facilitating mechanism could be operative at each step of resistance development.

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* Corresponding author. Mailing address: Department of Fundamental Microbiology, Quartier UNIL-Sorge, Biophore Building, CH-1015 Lausanne, Switzerland. Phone: 41.21.692.56.01/00. Fax: 41.21.692.56.05. E-mail: philippe.moreillon{at}unil.ch

Published ahead of print on 14 May 2007.

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Antimicrobial Agents and Chemotherapy, August 2007, p. 2774-2783, Vol. 51, No. 8
0066-4804/07/$08.00+0     doi:10.1128/AAC.01127-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Haenni, M., Moreillon, P. (2008). Fitness Cost and Impaired Survival in Penicillin-Resistant Streptococcus gordonii Isolates Selected in the Laboratory. Antimicrob. Agents Chemother. 52: 337-339 [Abstract] [Full Text]