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Antimicrobial Agents and Chemotherapy, September 2007, p. 3212-3216, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.01592-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Contribution of the CmeABC Efflux Pump to Macrolide and Tetracycline Resistance in Campylobacter jejuni{triangledown}

Amera Gibreel, Nicole M. Wetsch, and Diane E. Taylor*

Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Received 20 December 2006/ Returned for modification 10 March 2007/ Accepted 20 June 2007

We investigated the involvement of the CmeABC efflux pump in acquired resistance of Campylobacter jejuni to macrolides and tetracycline. Inactivation of the cmeB gene had no effect on macrolide resistance when all copies of the target gene carried an A2074C mutation. In contrast, the CmeABC pump significantly contributed to macrolide resistance when two or three copies of the 23S rRNA had an A2075G transition. Inactivation of the cmeB gene led to restoration of tetracycline susceptibility in the isolates examined. Complete susceptibility to tetracycline or macrolides, however, was not restored when phenylalanine-arginine ß-naphthylamide was used. These data confirm contribution of the CmeABC efflux pump to acquired resistance of Campylobacter jejuni to tetracycline and macrolides.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Phone: (780) 492-4777. Fax: (780) 492-7521. E-mail: diane.taylor{at}ualberta.ca

{triangledown} Published ahead of print on 2 July 2007.

Antimicrobial Agents and Chemotherapy, September 2007, p. 3212-3216, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.01592-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hannula, M., Hanninen, M.-L. (2008). Effect of putative efflux pump inhibitors and inducers on the antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coli. J Med Microbiol 57: 851-855 [Abstract] [Full Text]  


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