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Antimicrobial Agents and Chemotherapy, November 2008, p. 4141-4144, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.00511-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Macrolide Antibiotic-Mediated Downregulation of MexAB-OprM Efflux Pump Expression in Pseudomonas aeruginosa{triangledown}

Makoto Sugimura,1,{dagger} Hideaki Maseda,3 Hideaki Hanaki,2 and Taiji Nakae2*

Graduate School of Medical Science, Kitasato Institute University, 1-15-1 Kitasato, Sagamihara 228-8555, Japan,1 Laboratory for Anti-infectious Drugs, Kitasato Institute University, Bldg. S-105, 1-15-1 Kitasato, Sagamihara 228-8555, Japan,2 Department of Biological Science and Technology, Faculty of Engineering, 2-1 Minamijyousanjima-cho, Tokushima 770-8605, Japan3

Received 19 April 2008/ Returned for modification 2 June 2008/ Accepted 25 July 2008

Macrolide antibiotics modulate the quorum-sensing system of Pseudomonas aeruginosa. We tested the effect of macrolide antibiotics on the cell density-dependent expression of the MexAB-OprM efflux pump and found that 1.0 µg/ml (MIC/6.25) of azithromycin suppressed the expression of MexAB-OprM by about 70%, with the result that the cells became two- to fourfold more susceptible to antibiotics such as aztreonam, tetracycline, carbenicillin, chloramphenicol, and novobiocin.

* Corresponding author. Mailing address: Laboratory for Anti-infectious Drugs, Kitasato Institute University, Bldg. S-105, 1-15-1 Kitasato, Sagamihara 228-8555, Japan. Phone: 81-42-778-9932. Fax: 81-42-778-9931. E-mail: nakae-tj{at}insti.kitasato-u.ac.jp

{triangledown} Published ahead of print on 1 August 2008.

{dagger} Present address: Miroku Medical Laboratory, 659-2 Innai, Saku, Nagano 384-2201, Japan.

Antimicrobial Agents and Chemotherapy, November 2008, p. 4141-4144, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.00511-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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