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Antimicrobial Agents and Chemotherapy, December 2008, p. 4470-4474, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.00548-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Validation of ligA and tarI as Essential Targets in Staphylococcus aureus{triangledown}

Karin Streker,1,{dagger} Tina Schäfer,1,{dagger} Christoph Freiberg,2 Heike Brötz-Oesterhelt,3 Jörg Hacker,1,{ddagger} Harald Labischinski,4 and Knut Ohlsen1*

University of Würzburg, Institute for Molecular Infection Biology, Röntgenring 11, 97070 Würzburg,1 Bayer Healthcare AG, 42096 Wuppertal,2 AiCuris GmbH, Friedrich-Ebert-Strasse 475, D-42117 Wuppertal,3 Combinature Biopharm AG, Robert-Rössle-Str. 10, 13125 Berlin, Germany4

Received 25 April 2007/ Returned for modification 12 June 2007/ Accepted 28 August 2008

A conditional expression system has been developed using the isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove the essentiality of ligA and to evaluate the function of tarI, which was found to be essential in vitro but not in vivo.

* Corresponding author. Mailing address: Universität Würzburg, Institut für Molekulare Infektionsbiologie, Röntgenring 11, 97070 Würzburg, Germany. Phone: 49-931-312155. Fax: 49-931-312578. E-mail: knut.ohlsen{at}mail.uni-wuerzburg.de

{triangledown} Published ahead of print on 22 September 2008.

{ddagger} Present address: Robert-Koch-Institute, Nordufer 20, 13353 Berlin, Germany.

{dagger} K.S. and T.S. contributed equally to this work.

Antimicrobial Agents and Chemotherapy, December 2008, p. 4470-4474, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.00548-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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