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Antimicrobial Agents and Chemotherapy, December 2008, p. 4522-4525, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.00448-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In Vitro Activity of the Antifungal Plant Defensin RsAFP2 against Candida Isolates and Its In Vivo Efficacy in Prophylactic Murine Models of Candidiasis{triangledown}

Patricia M. Tavares,1 Karin Thevissen,2 Bruno P. A. Cammue,2 Isabelle E. J. A. François,2 Eliana Barreto-Bergter,1 Carlos P. Taborda,3 Alexandre F. Marques,3,{ddagger} Marcio L. Rodrigues,1,{dagger} and Leonardo Nimrichter1*,{dagger}

Laboratório de Estudos Integrados em Bioquímica Microbiana, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Cidade Universitária, CCS, Bloco I, Ilha do Fundão, Rio de Janeiro, RJ 21941590, Brazil,1 Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium,2 Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo, São Paulo, SP, Brazil3

Received 4 April 2008/ Returned for modification 24 May 2008/ Accepted 19 September 2008

We show that RsAFP2, a plant defensin that interacts with fungal glucosylceramides, is active against Candida albicans, inhibits to a lesser extent other Candida species, and is nontoxic to mammalian cells. Moreover, glucosylceramide levels in Candida species correlate with RsAFP2 sensitivity. We found RsAFP2 prophylactically effective against murine candidiasis.

* Corresponding author. Mailing address: Laboratorio de Estudos Integrados em Bioquimica Microbiana, Instituto de Microbiologia Professor Paulo de Goes, Universidade Federal do Rio de Janeiro, Cidade Universitaria, CCS, Bloco I, Ilha do Fundao, Rio de Janeiro, RJ 21941590, Brazil. Phone: 55 21 25626711. Fax: 55 21 25606344. E-mail: nimrichter{at}micro.ufrj.br

{triangledown} Published ahead of print on 29 September 2008.

{dagger} L.N. and M.L.R. share senior authorship on this article.

{ddagger} Present address: Department of Biological Sciences, University of Texas at El Paso, El Paso, TX.

Antimicrobial Agents and Chemotherapy, December 2008, p. 4522-4525, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.00448-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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