This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanaka, R.
Right arrow Articles by Kato, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, R.
Right arrow Articles by Kato, S.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2008, p. 782-785, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.01613-06
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Intracellular Efavirenz Levels in Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Individuals{triangledown}

Rie Tanaka,1 Hideji Hanabusa,2 Ei Kinai,2 Naoki Hasegawa,1 Masayoshi Negishi,1 and Shingo Kato1*

Keio University School of Medicine,1 Ogikubo Hospital, Tokyo, Japan2

Received 27 December 2006/ Returned for modification 15 March 2007/ Accepted 28 November 2007

We describe a novel method for isolating plasma-free peripheral blood mononuclear cells retaining intracellular efavirenz. Quantification of efavirenz in 13 human immunodeficiency virus-infected patients by liquid chromatography-tandem mass spectrometry showed a higher correlation of intracellular levels with unbound plasma levels (accumulation ratio, 1,190) than with total plasma levels.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. Phone: 81 3 5363 3769. Fax: 81 3 5360 1508. E-mail: skato{at}sc.itc.keio.ac.jp

{triangledown} Published ahead of print on 10 December 2007.

Antimicrobial Agents and Chemotherapy, February 2008, p. 782-785, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.01613-06
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Janneh, O., Chandler, B., Hartkoorn, R., Kwan, W. S., Jenkinson, C., Evans, S., Back, D. J., Owen, A., Khoo, S. H. (2009). Intracellular accumulation of efavirenz and nevirapine is independent of P-glycoprotein activity in cultured CD4 T cells and primary human lymphocytes. J Antimicrob Chemother 64: 1002-1007 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»