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Antimicrobial Agents and Chemotherapy, March 2008, p. 1201-1203, Vol. 52, No. 3
0066-4804/08/$08.00+0     doi:10.1128/AAC.00799-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Fluoroquinolone-Resistant Mutants of Burkholderia cepacia{triangledown}

C. F. Pope,1 S. H. Gillespie,1 J. R. Pratten,2 and T. D. McHugh1*

Centre for Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2QG,1 UCL Eastman Dental Institute, Gray's Inn Road, London WC1X 8LD, United Kingdom2

Received 20 June 2007/ Returned for modification 6 August 2007/ Accepted 16 December 2007

Fluoroquinolone-resistant Burkholderia cepacia mutants were selected on ciprofloxacin. The rate of mutation in gyrA was estimated to be 9.6 x 10–11 mutations per division. Mutations in gyrA conferred 12- to 64-fold increases in MIC, and an additional parC mutation conferred a large increase in MIC (>256-fold). Growth rate, biofilm formation, and survival in water and during drying were not impaired in strains containing single gyrA mutations. Double mutants were impaired only in growth rate (0.85, relative to the susceptible parent).

* Corresponding author. Mailing address: Centre for Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom. Phone: 44 20 7472 6402. Fax: 44 20 7794 0500, ext. 3540. E-mail: t.mchugh{at}medsch.ucl.ac.uk

{triangledown} Published ahead of print on 26 December 2007.

Antimicrobial Agents and Chemotherapy, March 2008, p. 1201-1203, Vol. 52, No. 3
0066-4804/08/$08.00+0     doi:10.1128/AAC.00799-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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