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Antimicrobial Agents and Chemotherapy, January 2009, p. 277-280, Vol. 53, No. 1
0066-4804/09/$08.00+0     doi:10.1128/AAC.00912-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Relative Strengths of the Class 1 Integron Promoter Hybrid 2 and the Combinations of Strong and Hybrid 1 with an Active P2 Promoter{triangledown}

Costas C. Papagiannitsis,1 Leonidas S. Tzouvelekis,2 and Vivi Miriagou1*

Laboratory of Bacteriology, Hellenic Pasteur Institute,1 Department of Microbiology, Medical School, University of Athens, Athens, Greece2

Received 10 July 2008/ Returned for modification 23 August 2008/ Accepted 2 November 2008

The relative strengths of the uncommon promoters hybrid 2, hybrid 1 with an active P2 promoter (hybrid 1+P2), and strong+P2, which drive transcription of resistance genes in class 1 integrons, were evaluated using blaGES-1 as a reporter gene cassette. Hybrid 2 was stronger than hybrid 1. Coupling P2 with the strong promoter and with hybrid 1 caused a measurable increase in GES-1 expression.

* Corresponding author. Mailing address: Laboratory of Bacteriology, Hellenic Pasteur Institute, Vas. Sofias 127, Athens 11521, Greece. Phone: 30-210-6478810. Fax: 30-210-6423626. E-mail: miriagou{at}pasteur.gr

{triangledown} Published ahead of print on 10 November 2008.

Antimicrobial Agents and Chemotherapy, January 2009, p. 277-280, Vol. 53, No. 1
0066-4804/09/$08.00+0     doi:10.1128/AAC.00912-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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