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Antimicrobial Agents and Chemotherapy, February 2009, p. 512-518, Vol. 53, No. 2
0066-4804/09/$08.00+0     doi:10.1128/AAC.00804-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Molecular Evidence for Spread of Two Major Methicillin-Resistant Staphylococcus aureus Clones with a Unique Geographic Distribution in Chinese Hospitals{triangledown}

Yudong Liu,1,2 Hui Wang,1,2* Na Du,3 Enhua Shen,3 Hongbin Chen,1,2 Junqi Niu,3 Huifen Ye,4 and Minjun Chen1

Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, People's Republic of China,1 Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, People's Republic of China,2 Department of Infectious Diseases, First Affiliated Hospital of Bethune Medical College, Jilin University, Changchun 130021, People's Republic of China,3 Department of Clinical Laboratory, Guangzhou First Municipal People's Hospital, Guangzhou 510180, People's Republic of China4

Received 19 June 2008/ Returned for modification 25 September 2008/ Accepted 14 November 2008

Methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) is a serious problem worldwide. To investigate the molecular epidemiology of MRSA isolates in China, a total of 702 MRSA isolates collected from 18 teaching hospitals in 14 cities between 2005 and 2006 were characterized by antibiogram analysis, pulsed-field gel electrophoresis (PFGE), staphylococcal cassette chromosome mec (SCCmec) typing, and spa typing; and 102 isolates were selected for multilocus sequence typing (MLST). Overall, SCCmec type III was the most popular type and was found in 541 isolates (77.1%), followed by SCCmec type II (109/702; 15.5%). Twenty-four PFGE types were obtained among 395 isolates collected in 2005, and 18 spa types were obtained among 702 isolates. spa type t030, which corresponded to PFEG types A to E, constituted 52.0% (365/702) of all isolates, and isolates of this type were present in all 14 cities; spa type t037, which corresponded to PFGE types F and G, accounted for 25.5% (179/702) of all isolates, and isolates of this type were identified in 12 cities. The two spa genotypes belonged to sequence type 239 (ST239) and carried SCCmec type III. spa type t002, which included isolates of PFGE types L to T, made up 16.0% (112/702) of the isolates that belonged to ST5 and SCCmec type II, and isolates of this type were distributed in 12 cities. The distribution of spa types varied among the regions. spa type t002 was the most common in Dalian (53.4%) and Shenyang (44.4%); spa type t037 was predominant in Shanghai (74.8%), whereas spa type t030 was the most common in the other cities. Two isolates from Guangzhou that harbored SCCmec type IVa with ST59 and ST88 were identified as community-associated MRSA. The prevalence of the Panton-Valentine leukocidin gene was 2.3%. The data documented two major epidemic MRSA clones, ST239-MRSA-SCCmec type III and ST5-MRSA-SCCmec type II, with unique geographic distributions across China.

* Corresponding author. Mailing address: Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Wangfujing, Beijing 100730, China. Phone and fax: 86-10-6529-5406. E-mail: wh_bj{at}tom.com

{triangledown} Published ahead of print on 24 November 2008.

Antimicrobial Agents and Chemotherapy, February 2009, p. 512-518, Vol. 53, No. 2
0066-4804/09/$08.00+0     doi:10.1128/AAC.00804-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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