This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vernel-Pauillac, F.
Right arrow Articles by Goarant, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vernel-Pauillac, F.
Right arrow Articles by Goarant, C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 2009, p. 1264-1267, Vol. 53, No. 3
0066-4804/09/$08.00+0     doi:10.1128/AAC.01104-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Quinolone Resistance in Neisseria gonorrhoeae: Rapid Genotyping of Quinolone Resistance-Determining Regions in gyrA and parC Genes by Melting Curve Analysis Predicts Susceptibility{triangledown}

Frédérique Vernel-Pauillac,1 Tiffany R. Hogan,2 John W. Tapsall,2 and Cyrille Goarant1*

Institut Pasteur de Nouvelle-Calédonie, Laboratoire de Recherche en Bactériologie, Nouméa Cedex, New Caledonia,1 WHO Collaborating Centre for STD, Department of Microbiology, The Prince of Wales Hospital, Sydney, Australia2

Received 18 August 2008/ Returned for modification 4 December 2008/ Accepted 26 December 2008

We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.

* Corresponding author. Mailing address: Institut Pasteur de Nouvelle-Calédonie, Laboratoire de Recherche en Bactériologie, Nouméa Cedex, New Caledonia. Phone: (687) 252666. Fax: (687) 273390. E-mail: cgoarant{at}pasteur.nc

{triangledown} Published ahead of print on 5 January 2009.

Antimicrobial Agents and Chemotherapy, March 2009, p. 1264-1267, Vol. 53, No. 3
0066-4804/09/$08.00+0     doi:10.1128/AAC.01104-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»