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Next Article 

Antimicrobial Agents and Chemotherapy, April 2009, p. 1299-1304, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.01358-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Engineered Streptomyces platensis Strains That Overproduce Antibiotics Platensimycin and Platencin{triangledown}

Michael J. Smanski,1 Ryan M. Peterson,2 Scott R. Rajski,2 and Ben Shen1,2,3,4*

Microbiology Doctoral Training Program,1 Division of Pharmaceutical Sciences,2 University of Wisconsin National Cooperative Drug Discovery Group,3 Department of Chemistry, University of Wisconsin—Madison, Madison, Wisconsin 537054

Received 9 October 2008/ Returned for modification 11 December 2008/ Accepted 8 January 2009

Platensimycin, which is isolated from Streptomyces platensis MA7327, and platencin, which is isolated from S. platensis MA7339, are two recently discovered natural products that serve as important antibiotic leads. Here we report on the identification of S. platensis MA7327 as a dual producer of both platensimycin and platencin. A PCR-based approach was used to locate and clone the locus involved in platensimycin and platencin production, including ptmR1, which encodes a putative GntR-like transcriptional regulator. Deletion of this gene from the producing organism allowed us to isolate strains that overproduce platensimycin and platencin with yields of 323 ± 29 mg/liter and 255 ± 30 mg/liter, respectively. These results illustrate the effectiveness of genetic manipulation for the rational engineering of improvements in titers.

* Corresponding author. Mailing address: Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin—Madison, 777 Highland Ave., Madison, WI 53705-2222. Phone: (608) 263-2673. Fax: (608) 262-5345. E-mail: bshen{at}pharmacy.wisc.edu

{triangledown} Published ahead of print on 21 January 2009.

Antimicrobial Agents and Chemotherapy, April 2009, p. 1299-1304, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.01358-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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