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Antimicrobial Agents and Chemotherapy, April 2009, p. 1586-1591, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.00940-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

In Vitro Activities of Retigeric Acid B Alone and in Combination with Azole Antifungal Agents against Candida albicans{triangledown}

Lingmei Sun,1 Shujuan Sun,2 Aixia Cheng,1 Xiuzhen Wu,1 Yu Zhang,1 and Hongxiang Lou1*

School of Pharmaceutical Sciences, Shandong University, No. 44 West Wenhua Road, Jinan, China,1 Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Jinan, China2

Received 16 July 2008/ Returned for modification 4 September 2008/ Accepted 16 January 2009

The vitro antifungal activity of retigeric acid B (RAB), a pentacyclic triterpenoid from the lichen species Lobaria kurokawae, was evaluated alone and in combination with fluconazole, ketoconazole, and itraconazole against Candida albicans using checkerboard microdilution and time-killing tests. The MICs for RAB against 10 different C. albicans isolates ranged from 8 to 16 µg/ml. A synergistic action of RAB and azole was observed in azole-resistant strains, whereas synergistic or indifferent effects were observed in azole-sensitive strains when interpreted by a separate approach of the fractional inhibitory concentration index and {Delta}E model (the difference between the predicted and measured fungal growth percentages). In time-killing tests, we used both colony counts and a colorimetric assay to evaluate the combinational antifungal effects of RAB and azoles, which further confirmed their synergistic interactions. These findings suggest that the natural product RAB may play a certain role in increasing the susceptibilities of azole-resistant C. albicans strains.

* Corresponding author. Mailing address: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, China. Phone: 86-531-88382012. Fax: 86-531-88382019. E-mail: louhongxiang{at}sdu.edu.cn

{triangledown} Published ahead of print on 26 January 2009.

Antimicrobial Agents and Chemotherapy, April 2009, p. 1586-1591, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.00940-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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