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Antimicrobial Agents and Chemotherapy, June 2009, p. 2679-2683, Vol. 53, No. 6
0066-4804/09/$08.00+0     doi:10.1128/AAC.00063-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Analysis of blaSHV Codon 238 and 240 Allele Mixtures Using Sybr Green High-Resolution Melting Analysis{triangledown}

Patiyan Andersson, Tegan Harris, Steven Y. C. Tong, and Philip M. Giffard*

Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia

Received 15 January 2009/ Returned for modification 15 February 2009/ Accepted 27 March 2009

Klebsiella pneumoniae isolates frequently contain complex mixtures of blaSHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum β-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.

* Corresponding author. Mailing address: Menzies School of Health Research, Royal Darwin Hospital Campus, Casuarina, Northern Territory 0810, Australia. Phone: 61 8 8944 8040. Fax: 61 8 8927 5187. E-mail: phil.giffard{at}menzies.edu.au

{triangledown} Published ahead of print on 6 April 2009.

Antimicrobial Agents and Chemotherapy, June 2009, p. 2679-2683, Vol. 53, No. 6
0066-4804/09/$08.00+0     doi:10.1128/AAC.00063-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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