This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Doi, Y.
Right arrow Articles by Bonomo, R. A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doi, Y.
Right arrow Articles by Bonomo, R. A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2009, p. 3159-3161, Vol. 53, No. 7
0066-4804/09/$08.00+0     doi:10.1128/AAC.00133-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Reduced Susceptibility to Cefepime among Escherichia coli Clinical Isolates Producing Novel Variants of CMY-2 β-Lactamase {triangledown}

Yohei Doi,1* David L. Paterson,1,2 Jennifer M. Adams-Haduch,1 Hanna E. Sidjabat,1 Alexandra O'Keefe,1 Andrea Endimiani,3,4 and Robert A. Bonomo3,4,5,6

Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania,1 Centre for Clinical Research, University of Queensland, Royal Brisbane and Women's Hospital, Brisbane, Australia,2 Research Services, Louis Stokes Cleveland Department of Veteran Affairs Medical Center, Cleveland, Ohio,3 Departments of Medicine,4 Pharmacology,5 Molecular Biology and Microbiology, Case School of Medicine, Cleveland, Ohio6

Received 29 January 2009/ Returned for modification 14 March 2009/ Accepted 26 April 2009

Here we describe three Escherichia coli clinical isolates with reduced susceptibility to cefepime. Sequencing of the blaCMY genes revealed two novel variants (CMY-33 and -44) with two- to four-amino-acid deletions in the H-10 helix. The deletions were responsible for 12- to 24-fold increases in the MICs of cefepime.

* Corresponding author. Mailing address: Division of Infectious Diseases, University of Pittsburgh Medical Center, S829 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261. Phone: (412) 648-9445. Fax: (412) 648-8521. E-mail: yod4{at}pitt.edu

{triangledown} Published ahead of print on 4 May 2009.

Antimicrobial Agents and Chemotherapy, July 2009, p. 3159-3161, Vol. 53, No. 7
0066-4804/09/$08.00+0     doi:10.1128/AAC.00133-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»