AAC Accepts, published online ahead of print on 6 April 2009

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Antimicrob. Agents Chemother. doi:10.1128/AAC.00063-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Analysis of bla_SHV codon 238 and 240 allele mixtures using SYBR Green high resolution melting analysis

Patiyan Andersson, Tegan Harris, Steven Y.C. Tong, and Philip M. Giffard^*

Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia

^* To whom correspondence should be addressed. Email: phil.giffard{at}menzies.edu.au.

Klebsiella pneumoniae isolates frequently contain complex mixes of bla_SHV alleles. A high resolution melting based method for interrogating the extended spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended spectrum -lactamase encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.