AAC Accepts, published online ahead of print on 3 March 2008

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Antimicrob. Agents Chemother. doi:10.1128/AAC.00110-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Pyrazinamide Resistance and pncA Gene Mutations in Mycobacterium tuberculosis

Pontus Juréen^*, Jim Werngren, Juan-Carlos Toro, and Sven Hoffner

Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, and Department of Microbiology, Tumour and Cellbiology Center, Karolinska Institute, Stockholm, Sweden

^* To whom correspondence should be addressed. Email: pontus.jureen{at}smi.ki.se.

Thirty-four pyrazinamide resistant and 37 susceptible M. tuberculosis-complex strains were analyzed for pncA gene mutations. None of the sensitive strains had any mutations, apart from silent mutations, whereas all but one resistant strain showed pncA mutations. By using sequencing as a means of early resistance detection, the inconsistency of phenotypic pyrazinamide assays can be circumvented.

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