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AAC Accepts, published online ahead of print on 18 June 2007
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Antimicrob. Agents Chemother. doi:10.1128/AAC.00275-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Update to the multiplex PCR strategy for the assignment of mec element types in Staphylococcus aureus

Catarina Milheiriço, Duarte C. Oliveira*, and Hermínia de Lencastre

Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal; Laboratory of Microbiology, The Rockefeller University, New York, NY 10021

* To whom correspondence should be addressed. Email: dco{at}itqb.unl.pt.


   Abstract

SCCmec typing is important for the identification and definition of MRSA clones and, for routine purposes, multiplex PCR assays are the most adequate. Here, we describe an update to the multiplex PCR strategy for SCCmec typing we described in 2002, so that SCCmec types IV and V are properly identified.




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