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Antimicrob. Agents Chemother. doi:10.1128/AAC.00355-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A molecular basis for the resistance of Acanthamoeba tubulins to all major classes of anti-tubulin compounds

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow, Scotland, UK, G4 0NR; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK, S10 2TN

^* To whom correspondence should be addressed. Email: fiona.henriquez{at}paisley.ac.uk.

	Abstract

Tubulin is essential to eukaryotic cells and is targeted by several anti-neoplastics, herbicides and antimicrobials. We demonstrate that Acanthamoeba are resistant to five anti-microtubule compounds, unlike any other eukaryote studied so far. Resistance correlates with critical amino acid differences within the inhibitor binding sites of the tubulin heterodimers.