AAC Accepts, published online ahead of print on 14 July 2008

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ruzin, A. Articles by Bradford, P. A. Search for Related Content PubMed PubMed Citation Articles by Ruzin, A. Articles by Bradford, P. A.

 Previous Article  |  Next Article 

Antimicrob. Agents Chemother. doi:10.1128/AAC.00591-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

RT-PCR and statistical analyses of acrAB and ramA expression in clinical isolates of Klebsiella pneumoniae

Alexey Ruzin^*, Frederick W. Immermann, and Patricia A. Bradford

Wyeth Research, Infectious Disease, Pearl River, New York, 10965; Wyeth Research, Translational Medicine Biostatistics, Pearl River, New York, 10965

^* To whom correspondence should be addressed. Email: ruzina{at}wyeth.com.

Clinical isolates of Klebsiella pneumoniae were tested for a correlation between tigecycline MIC and expression of ramA using RT-PCR. At MICs of 4 and 8 µg/ml, the expression of ramA was statistically significantly different from MICs of 2 µg/ml or less supporting the tigecycline susceptibility breakpoint of 2 µg/ml for K. pneumoniae.

This article has been cited by other articles:

• Bratu, S., Landman, D., George, A., Salvani, J., Quale, J. (2009). Correlation of the expression of acrB and the regulatory genes marA, soxS and ramA with antimicrobial resistance in clinical isolates of Klebsiella pneumoniae endemic to New York City. J Antimicrob Chemother 64: 278-283 [Abstract] [Full Text]