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Antimicrob. Agents Chemother. doi:10.1128/AAC.00629-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Therapeutic effect of nisin Z on subclinical mastitis in lactating cows

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University Hangzhou 310029, China

^* To whom correspondence should be addressed. Email: Songhua{at}zju.edu.cn.

	Abstract

Bovine subclinical mastitis is the inflammation of mammary gland caused by bacterial intramammary infection, accounting for large amount of economic loss. Treatment of subclinical mastitis is not suggested in lactating cows due to the risk of milk contamination. Objectives of this study were to evaluate an antimicrobial peptide, nisin, in the treatment of subclinical mastitis in lactating cows. A total of 90 Holstein lactating cows with subclinical mastitis were randomly divided into nisin-treated (n = 46) and control (n = 44) groups. In nisin-treated group, cows received an intramammary infusion of nisin at a dose of 2,500,000 IU once daily for 3 days while the control cows received no treatment. Milk samples were collected from the affected mammary quarters before initiating, 1 and 2 weeks after treatments for analyses of bacteria, somatic cells and N-acetyl--D-glucosaminidase (NAGase). Results indicated that nisin therapy caused bacteriological cure rate of 90.1% for Streptococcus agalactiae (10 of 11), 50% for Staphylococcus aureus (7 of 14), 58.8% for coagulase negative staphylococci (7 of 17), and 65.2% for all cases (30 of 46). Meanwhile, only 15.9% (7 of 44) spontaneously recovered in the no-treated cows. NAGase activiy in milk samples and the number of mammary quarters with milk SCC 500,000/mL were significantly decreased after nisin-treatment while no significant changes took place in the control group. Because of its therapeutic effects on bovine subclinical mastitis, as well as its safety in humans, nisin deserves further study to clarify its effects on different pathogen caused mastitis.

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