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Antimicrob. Agents Chemother. doi:10.1128/AAC.00717-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

BIOSYNTHETIC INVESTIGATIONS OF LACTONAMYCIN AND LACTONAMYCIN Z: CLONING OF THE BIOSYNTHETIC GENE CLUSTERS AND DISCOVERY OF AN UNUSUAL STARTER UNIT

Department of Chemistry MS60, Rice University, 6100 Main St., Houston, TX 77005; Mikrobiologisches Institut, Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tubingen, Germany; School of Biology, University of Newcastle, Newcastle Upon Tyne, NE1 7RU, United Kingdom

^* To whom correspondence should be addressed. Email: parry{at}rice.edu.

	Abstract

The antibiotics lactonamycin and lactonamycin Z provide attractive leads for antibacterial drug development. Both antibiotics contain a novel aglycone core called lactonamycinone. To gain insight into lactonamycinone biosynthesis, cloning and precursor incorporation experiments were undertaken. The lactonamycin gene cluster was initially cloned from Streptomyces rishiriensis. Sequencing of ca. 61 kb of S. rishiriensis DNA revealed the presence of 57 open reading frames (ORFs). These included genes coding for the biosynthesis of L-rhodinose, the sugar found in lactonamycin, and genes similar to those in the tetracenomycin biosynthetic gene cluster. Since lactonamycin production by S. rishiriensis could not be sustained, additional proof for the identity of the S. rishiriensis cluster was obtained by cloning the lactonamycin Z gene cluster from S. sanglieri. Partial sequencing of the S. sanglieri cluster revealed fifteen genes that exhibited a very high degree of similarity to genes within the lactonamycin cluster, as well as an identical organization. Double-crossover disruption of one gene in the S. sanglieri cluster abolished lactonamycin Z production, and production was restored by complementation. These results confirm the identity of the genetic locus cloned from S. sanglieri and indicate that the highly similar locus in S. rishiriensis encodes lactonamycin biosynthetic genes. Precursor incorporation experiments with S. sanglieri revealed that lactonamycinone is biosynthesized in an unusual manner whereby glycine or a glycine derivative serves as a starter unit that is extended by nine acetate units. Analysis of the gene clusters and of the precursor incorporation data suggested a hypothetical scheme for lactonamycinone biosynthesis.