This Article Full Text (PDF) Other Versions of this Article: AAC.01374-06v1 51/4/1487    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zolezzi, P. C. Articles by Gómez-Lus, R. Search for Related Content PubMed PubMed Citation Articles by Zolezzi, P. C. Articles by Gómez-Lus, R.

 Previous Article  |  Next Article 

Antimicrob. Agents Chemother. doi:10.1128/AAC.01374-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Molecular epidemiology of macrolide and tetracycline resistances in commensal Gemella spp. isolates

Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza. C/Domingo Miral s/n, 50009 Zaragoza, Centro de Salud Internacional, IDIBAPS, Hospital Clinic. Villarroel 170, 08036 Barcelona and Service of Microbiology, Hospital Clínico Lozano Blesa. C/San Juan Bosco 19, 50009 Zaragoza, Spain

^* To whom correspondence should be addressed. Email: gomezlus{at}unizar.es.

	Abstract

The epidemiologic relatedness of twenty nine erythromycin-resistant Gemella spp. from normal flora, characterized previously, were evaluated by pulsed-field gel electrophoresis. Three isolates carried the tet(O) and the tet(M) gene. The msr(A) gene was found in two Gemella morbillorum strains in combination with the erm(B) or the mef(E) gene. Sequences of the mef(A/E), erm(B) and msr(A) genes showed a high similarity to the corresponding sequences of other gram positive cocci. All the strains harbouring the mef(A/E) and the msr(D) gene possessed the ORF3/ORF6. The 16 G. morbillorum isolates represented 15 distinct DNA profiles. Four clusters were identified ( 80% of genetic relatedness). The 12 G. haemolysans strains belonged to different PFGE types. The clonal diversity found suggest that horizontal transfer may be the main route through which erythromycin resistance is acquired.

This article has been cited by other articles:

• Sekirov, I., Tam, N. M., Jogova, M., Robertson, M. L., Li, Y., Lupp, C., Finlay, B. B. (2008). Antibiotic-Induced Perturbations of the Intestinal Microbiota Alter Host Susceptibility to Enteric Infection. Infect. Immun. 76: 4726-4736 [Abstract] [Full Text]