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Research Article

Biosynthetic studies on antibiotic A47934.

M J Zmijewski, Jr, B Briggs, R Logan, L D Boeck
M J Zmijewski
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285-1533.
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B Briggs
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285-1533.
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R Logan
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285-1533.
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L D Boeck
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285-1533.
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DOI: 10.1128/AAC.31.10.1497
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ABSTRACT

A47934, a peptide antibiotic produced by Streptomyces toyocaensis, belongs to the glycopeptide class of compounds which includes ristocetin and vancomycin. Incorporation studies with radioisotope-labeled substrates indicated that tyrosine, p-hydroxyphenylglycine, p-hydroxyphenylglyoxylate, acetate, and sulfate were efficiently incorporated into A47934. This is consistent with the reported biosynthesis of other glycopeptide antibiotics. Prototrophic mutants blocked in antibiotic biosynthesis were isolated at a low frequency (0.4%) after mutagenesis. Secretor-convertor pairings of the 36 mutants obtained demonstrated that they belonged to three classes: two groups of secretor-convertor pairs and a larger group of mutants that did not make antibiotic under any condition tested. Neither the secretor-convertor studies not supplementation of the cultures with putative biosynthetic intermediates was useful in identifying the location of the biosynthetic blocks. All studies to determine the timing of the sulfate addition step in the biosynthesis indicated that the sulfate is added prior to the formation of intermediates that possess antimicrobial activity.

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Biosynthetic studies on antibiotic A47934.
M J Zmijewski Jr, B Briggs, R Logan, L D Boeck
Antimicrobial Agents and Chemotherapy Oct 1987, 31 (10) 1497-1501; DOI: 10.1128/AAC.31.10.1497

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Biosynthetic studies on antibiotic A47934.
M J Zmijewski Jr, B Briggs, R Logan, L D Boeck
Antimicrobial Agents and Chemotherapy Oct 1987, 31 (10) 1497-1501; DOI: 10.1128/AAC.31.10.1497
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Print ISSN: 0066-4804; Online ISSN: 1098-6596