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Comparative Study | Journal Article | Research Support, Non-U.S. Gov't

Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi.

A Espinel-Ingroff, K Dawson, M Pfaller, E Anaissie, B Breslin, D Dixon, A Fothergill, V Paetznick, J Peter, M Rinaldi
A Espinel-Ingroff
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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K Dawson
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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M Pfaller
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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E Anaissie
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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B Breslin
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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D Dixon
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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A Fothergill
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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V Paetznick
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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J Peter
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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M Rinaldi
Medical College of Virginia/Virginia Commonwealth University, Richmond, USA.
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DOI: 10.1128/AAC.39.2.314
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ABSTRACT

The purpose of the study was to evaluate the interlaboratory agreement of broth dilution susceptibility methods for five species of conidium-forming (size range, 2 to 7 microns) filamentous fungi. The methods used included both macro- and microdilution methods that were adaptations of the proposed reference method of the National Committee for Clinical Laboratory Standards for yeasts (m27-P). The MICs of amphotericin B, fluconazole, itraconazole, miconazole, and ketoconazole were determined in six centers by both macro- and microdilution tests for 25 isolates of Aspergillus flavus, Aspergillus fumigatus, Pseudallescheria boydii, Rhizopus arrhizus, and Sporothrix schenckii. All isolates produced clearly detectable growth within 1 to 4 days at 35 degrees C in the RPMI 1640 medium. Colony counts of 0.4 x 10(6) to 3.3 x 10(6) CFU/ml (mean, 1.4 x 10(6) CFU/ml) were demonstrated in 90% of the 148 inoculum preparations. Overall, good intralaboratory agreement was demonstrated with amphotericin B, fluconazole, and ketoconazole MICs (90 to 97%). The agreement was lower with itraconazole MICs (59 to 79% median). Interlaboratory reproducibility demonstrated similar results: 90 to 100% agreement with amphotericin B, fluconazole, miconazole, and ketoconazole MICs and 59 to 91% with itraconazole MICs. Among the species tested, the MICs for S. schenckii showed the highest variability. The results of the study imply that it may be possible to develop a reference method for antifungal susceptibility testing of filamentous fungi.

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Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi.
A Espinel-Ingroff, K Dawson, M Pfaller, E Anaissie, B Breslin, D Dixon, A Fothergill, V Paetznick, J Peter, M Rinaldi
Antimicrobial Agents and Chemotherapy Feb 1995, 39 (2) 314-319; DOI: 10.1128/AAC.39.2.314

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Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi.
A Espinel-Ingroff, K Dawson, M Pfaller, E Anaissie, B Breslin, D Dixon, A Fothergill, V Paetznick, J Peter, M Rinaldi
Antimicrobial Agents and Chemotherapy Feb 1995, 39 (2) 314-319; DOI: 10.1128/AAC.39.2.314
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