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Journal Article

New extended-spectrum TEM-type beta-lactamase from Salmonella enterica subsp. enterica isolated in a nosocomial outbreak.

M I Morosini, R Canton, J Martinez-Beltran, M C Negri, J C Perez-Diaz, F Baquero, J Blazquez
M I Morosini
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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R Canton
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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J Martinez-Beltran
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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M C Negri
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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J C Perez-Diaz
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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F Baquero
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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J Blazquez
Department of Microbiology, Hospital Ramón y Cajal, National Institute of Health (INSALUD), Madrid, Spain.
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DOI: 10.1128/AAC.39.2.458
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ABSTRACT

A new extended-spectrum beta-lactamase was detected in a lactose-positive Salmonella enterica subsp. enterica strain that caused a nosocomial outbreak involving eight patients in a pediatric cardiology unit. This strain showed high levels of resistance to ceftazidime and aztreonam and relatively low levels of resistance to cefotaxime and ceftriaxone. Resistance was associated with a conjugative plasmid of 59 kb, which encoded a new beta-lactamase with an isoelectric point of 5.9 that strongly hydrolyzed ceftazidime and to a much lesser extent hydrolyzed cefotaxime. The enzyme activity was inhibited by clavulanate. The corresponding bla gene was cloned and sequenced. The deduced amino acid sequence showed three significant amino acid replacements with respect to the TEM-1 sequence: Arg-164-->His, Glu-240-->Lys, and Thr-265-->Met. This combination is unique among extended-spectrum beta-lactamases and served to characterize the new enzyme, TEM-27.

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New extended-spectrum TEM-type beta-lactamase from Salmonella enterica subsp. enterica isolated in a nosocomial outbreak.
M I Morosini, R Canton, J Martinez-Beltran, M C Negri, J C Perez-Diaz, F Baquero, J Blazquez
Antimicrobial Agents and Chemotherapy Feb 1995, 39 (2) 458-461; DOI: 10.1128/AAC.39.2.458

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New extended-spectrum TEM-type beta-lactamase from Salmonella enterica subsp. enterica isolated in a nosocomial outbreak.
M I Morosini, R Canton, J Martinez-Beltran, M C Negri, J C Perez-Diaz, F Baquero, J Blazquez
Antimicrobial Agents and Chemotherapy Feb 1995, 39 (2) 458-461; DOI: 10.1128/AAC.39.2.458
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