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Susceptibility

Antimicrobial Susceptibility of Haemophilus influenzae in the Respiratory Tracts of Patients with Cystic Fibrosis

Lieke V. M. Möller, Annette G. Regelink, Henny Grasselier, Loek van Alphen, Jacob Dankert
Lieke V. M. Möller
Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam, and
Department of Medical Microbiology, University Hospital Groningen and Laboratory for Public Health, Groningen, The Netherlands
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Annette G. Regelink
Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam, and
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Henny Grasselier
Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam, and
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Loek van Alphen
Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam, and
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Jacob Dankert
Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam, and
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DOI: 10.1128/AAC.42.2.319
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ABSTRACT

We analyzed the antimicrobial susceptibilities of Haemophilus influenzae isolates from 157 sputum specimens prospectively collected from 39 cystic fibrosis (CF) patients during a 2-year study. These isolates were characterized by random amplified polymorphic DNA analysis and major outer membrane protein (MOMP) analysis to identify H. influenzae strains and MOMP variants and to assess their persistence in the respiratory tract. Among the 247H. influenzae isolates, 16 (6.5%) produced β-lactamase. The 231 β-lactamase-negative isolates represented 85H. influenzae strains, 61 MOMP variants derived from 27 of these strains, and 85 persistent isolates identical to strains or MOMP variants. All β-lactamase-negative isolates were tested for susceptibility to ampicillin, amoxicillin-clavulanic acid, cefuroxime, cefotaxime, cefaclor, imipenem, tetracycline, and trimethoprim-sulfamethoxazole by disk diffusion testing. Eleven (13%)H. influenzae strains, 18 (30%) MOMP variants, and 30 (35%) persistent isolates were resistant to one or more of the antibiotics tested. Antimicrobial susceptibility was decreased among MOMP variants and persistent isolates compared to nonpersistentH. influenzae strains, and changes in susceptibility occurred irrespective of MOMP variation. We conclude that the decreased antimicrobial susceptibility of H. influenzae during persistence contributes to the poor eradication of H. influenzae from the respiratory tracts of CF patients.

  • Copyright © 1998 American Society for Microbiology
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Antimicrobial Susceptibility of Haemophilus influenzae in the Respiratory Tracts of Patients with Cystic Fibrosis
Lieke V. M. Möller, Annette G. Regelink, Henny Grasselier, Loek van Alphen, Jacob Dankert
Antimicrobial Agents and Chemotherapy Feb 1998, 42 (2) 319-324; DOI: 10.1128/AAC.42.2.319

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Antimicrobial Susceptibility of Haemophilus influenzae in the Respiratory Tracts of Patients with Cystic Fibrosis
Lieke V. M. Möller, Annette G. Regelink, Henny Grasselier, Loek van Alphen, Jacob Dankert
Antimicrobial Agents and Chemotherapy Feb 1998, 42 (2) 319-324; DOI: 10.1128/AAC.42.2.319
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KEYWORDS

Anti-Bacterial Agents
cystic fibrosis
Haemophilus influenzae
Respiratory System

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