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Mechanisms of Resistance

Cefotaxime-Resistant EnterobacteriaceaeIsolates from a Hospital in Warsaw, Poland: Identification of a New CTX-M-3 Cefotaxime-Hydrolyzing β-Lactamase That Is Closely Related to the CTX-M-1/MEN-1 Enzyme

Marek Gniadkowski, Ines Schneider, Andrzej Pal/ucha, Renate Jungwirth, Barbara Mikiewicz, Adolf Bauernfeind
Marek Gniadkowski
Sera and Vaccines Central Research Laboratory, 00-725 Warsaw, and
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Ines Schneider
Max von Pettenkofer-Institut, 80336 Munich, Germany
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Andrzej Pal/ucha
Sera and Vaccines Central Research Laboratory, 00-725 Warsaw, and
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Renate Jungwirth
Max von Pettenkofer-Institut, 80336 Munich, Germany
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Barbara Mikiewicz
Health Care Center Praga-Pól/noc, 03-719 Warsaw, Poland, and
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Adolf Bauernfeind
Max von Pettenkofer-Institut, 80336 Munich, Germany
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DOI: 10.1128/AAC.42.4.827
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ABSTRACT

A group of cefotaxime-resistant Citrobacter freundiiand Escherichia coli isolates were collected by a clinical laboratory in a hospital in Warsaw, Poland, in July 1996. Detailed analysis has shown that all of these produced a β-lactamase (pI, 8.4) belonging to the CTX-M family, one of the minor extended-spectrum β-lactamase families with a strong cefotaxime-hydrolyzing activity. Sequencing has revealed that C. freundii isolates produced a new CTX-M-3 enzyme which is very closely related to the CTX-M-1/MEN-1 β-lactamase, sporadically identified in Europe over a period of 6 years. Amino acid sequences of these two β-lactamases differ at four positions: Val77Ala, Asp114Asn, Ser140Ala, and Asn288Asp (the first amino acid of each pair refers to CTX-M-1/MEN-1 and second refers to CTX-M-3). The partial sequence of the E. coli CTX-M gene was identical to the corresponding region ofblaCTX-M-3, but a transconjugant of theE. coli isolate expressed higher levels of resistance to β-lactams than did C. freundii transconjugants. These resistance differences correlated with differences in plasmid DNA restriction patterns. Our results suggest that CTX-M genes have been spread among different species of the familyEnterobacteriaceae in the hospital and that the CTX-M-3-expressing C. freundii strain causing routine urinary tract infections has been maintained for a relatively long time in the hospital environment.

  • Copyright © 1998 American Society for Microbiology
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Cefotaxime-Resistant EnterobacteriaceaeIsolates from a Hospital in Warsaw, Poland: Identification of a New CTX-M-3 Cefotaxime-Hydrolyzing β-Lactamase That Is Closely Related to the CTX-M-1/MEN-1 Enzyme
Marek Gniadkowski, Ines Schneider, Andrzej Pal/ucha, Renate Jungwirth, Barbara Mikiewicz, Adolf Bauernfeind
Antimicrobial Agents and Chemotherapy Apr 1998, 42 (4) 827-832; DOI: 10.1128/AAC.42.4.827

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Cefotaxime-Resistant EnterobacteriaceaeIsolates from a Hospital in Warsaw, Poland: Identification of a New CTX-M-3 Cefotaxime-Hydrolyzing β-Lactamase That Is Closely Related to the CTX-M-1/MEN-1 Enzyme
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Cefotaxime-Resistant EnterobacteriaceaeIsolates from a Hospital in Warsaw, Poland: Identification of a New CTX-M-3 Cefotaxime-Hydrolyzing β-Lactamase That Is Closely Related to the CTX-M-1/MEN-1 Enzyme
Marek Gniadkowski, Ines Schneider, Andrzej Pal/ucha, Renate Jungwirth, Barbara Mikiewicz, Adolf Bauernfeind
Antimicrobial Agents and Chemotherapy Apr 1998, 42 (4) 827-832; DOI: 10.1128/AAC.42.4.827
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KEYWORDS

cefotaxime
Cephalosporin Resistance
cephalosporins
Enterobacteriaceae
Enterobacteriaceae Infections
beta-lactamases

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