Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Antimicrobial Agents and Chemotherapy
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About AAC
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • AAC Podcast
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mechanisms of Resistance

Effect of Growth Rate on Resistance ofCandida albicans Biofilms to Antifungal Agents

George S. Baillie, L. Julia Douglas
George S. Baillie
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
L. Julia Douglas
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/AAC.42.8.1900
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Elution of C. albicans from a cellulose acetate filter perfused with medium in the biofilm fermentor at a rate of 1.12 ml min−1. Organisms eluted during the initial 80 min correspond to loosely attached cells. Thereafter, newly formed daughter cells are eluted under steady-state conditions. The results are from a representative experiment repeated at least three times.

  • Fig. 2.
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Determination of the critical medium flow rate for aC. albicans biofilm in the perfused biofilm fermentor. The number of daughter cells released from the biofilm increased with flow up to 1.7 ml min−1. This represents the critical medium flow rate at which the growth rate is maximum (μmax). The results are from a typical experiment repeated at least three times.

  • Fig. 3.
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Relationship between flow rate and growth rate for aC. albicans biofilm in the perfused biofilm fermentor up to the critical medium flow rate (1.7 ml min−1).

  • Fig. 4.
    • Open in new tab
    • Download powerpoint
    Fig. 4.

    Scanning electron micrograph of a C. albicans biofilm grown on a cellulose acetate filter in a perfused biofilm fermentor. The growth rate was 0.2 h−1. Bar, 10 μm.

  • Fig. 5.
    • Open in new tab
    • Download powerpoint
    Fig. 5.

    Perfusion of C. albicans biofilms with antifungal agents. Biofilms were maintained at a growth rate of 0.2 h−1 and were perfused with medium containing amphotericin B (○), flucytosine (■), ketoconazole (□), fluconazole (▴), or no antifungal agent (•). The antifungal agents were used at the following concentrations (20 times the MIC): amphotericin B, 26 μg ml−1; flucytosine, 4 μg ml−1; fluconazole, 8 μg ml−1; and ketoconazole, 0.5 μg ml−1. Results represent mean values from two independent experiments carried out with duplicate samples. SEs were less than 10% of the mean values. The viable counts of eluates from biofilms perfused with amphotericin B were zero after 225 min.

  • Fig. 6.
    • Open in new tab
    • Download powerpoint
    Fig. 6.

    Perfusion of C. albicans biofilms grown at different rates with amphotericin B. Biofilms were maintained at a growth rate of 0.02 h−1 (○), 0.2 h−1 (■), or 0.4 h−1 (□). Control biofilms (•) were grown at a rate of 0.2 h−1 in medium without amphotericin B. Results represent mean values from two independent experiments carried out with duplicate samples. SEs were less than 10% of the mean values.

  • Fig. 7.
    • Open in new tab
    • Download powerpoint
    Fig. 7.

    Survival of planktonic and biofilm cells ofC. albicans grown at different rates after treatment with amphotericin B. Intact biofilms (□), resuspended biofilm cells (■), biofilm daughter cells (○), and planktonic cells (•) were exposed to amphotericin B for 1 h, and the percent survival was estimated by determining the viable counts. Results represent mean values (± standard error of the mean) from two independent experiments with viable counts determined in triplicate.

PreviousNext
Back to top
Download PDF
Citation Tools
Effect of Growth Rate on Resistance ofCandida albicans Biofilms to Antifungal Agents
George S. Baillie, L. Julia Douglas
Antimicrobial Agents and Chemotherapy Aug 1998, 42 (8) 1900-1905; DOI: 10.1128/AAC.42.8.1900

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Antimicrobial Agents and Chemotherapy article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Effect of Growth Rate on Resistance ofCandida albicans Biofilms to Antifungal Agents
(Your Name) has forwarded a page to you from Antimicrobial Agents and Chemotherapy
(Your Name) thought you would be interested in this article in Antimicrobial Agents and Chemotherapy.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Effect of Growth Rate on Resistance ofCandida albicans Biofilms to Antifungal Agents
George S. Baillie, L. Julia Douglas
Antimicrobial Agents and Chemotherapy Aug 1998, 42 (8) 1900-1905; DOI: 10.1128/AAC.42.8.1900
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

antifungal agents
biofilms
Candida albicans

Related Articles

Cited By...

About

  • About AAC
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • AAC Podcast
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #AACJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0066-4804; Online ISSN: 1098-6596