DOI: 10.1128/AAC.43.1.157
Article Figures & Data
Figures
- Fig. 1.
Nucleotide sequence and predicted amino acid sequence of the plasmid-mediated aadA-1 gene of E. faecalis W4770. The 1,009-bp PCR product was amplified with oligonucleotide primers specific for the integron cassette described by Lévesque et al. (21, 22). The gene insertion sites (GTTA) for the integron, at the 3′ end of each of the two shaded areas which highlight the hs2 (14 bp) and hs1 (55 bp) hot spots; the initiation and stop codons are in boldface type; and the 59-bp element is underlined (28).
Tables
- Table 1.
PCR amplification of aminoglycoside resistance genes
Gene(s) Primer pair (5′→3′)a Product size (bp) Refer-ence aadE (1268) ACT GGC TTA ATC AAT TTG GG 597 13 (1845) GCC TTT CCG CCA CCT CAC CG aadA (564)b TGA TTT GCT GGT TAC GGT GAC 284 16 (829)b CGC TAT GTT CTC TTG CTT TTG aac(6′)-aph(2") (348) TGA TGA TTT TCC TTT GAT GT 1,395 14 (1723) CAA TCT TTA TAA GTC CTT TT
