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Mechanisms of Resistance

Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis

Nancye C. Clark, Ørjan Olsvik, Jana M. Swenson, Carol A. Spiegel, Fred C. Tenover
Nancye C. Clark
Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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Ørjan Olsvik
Department of Medical Microbiology, School of Medicine, University of Tromsø, Tromsø, Norway; and
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Jana M. Swenson
Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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Carol A. Spiegel
Department of Pathology and Laboratory Medicine, University of Wisconsin Hospital and Clinics, Madison, Wisconsin 53792
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Fred C. Tenover
Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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DOI: 10.1128/AAC.43.1.157
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    Fig. 1.

    Nucleotide sequence and predicted amino acid sequence of the plasmid-mediated aadA-1 gene of E. faecalis W4770. The 1,009-bp PCR product was amplified with oligonucleotide primers specific for the integron cassette described by Lévesque et al. (21, 22). The gene insertion sites (GTTA) for the integron, at the 3′ end of each of the two shaded areas which highlight the hs2 (14 bp) and hs1 (55 bp) hot spots; the initiation and stop codons are in boldface type; and the 59-bp element is underlined (28).

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    PCR amplification of aminoglycoside resistance genes

    Gene(s)Primer pair (5′→3′)aProduct size (bp)Refer-ence
    aadE(1268) ACT GGC TTA ATC AAT TTG GG59713
    (1845) GCC TTT CCG CCA CCT CAC CG
    aadA(564)b TGA TTT GCT GGT TAC GGT GAC28416
    (829)b CGC TAT GTT CTC TTG CTT TTG
    aac(6′)-aph(2")(348) TGA TGA TTT TCC TTT GAT GT1,39514
    (1723) CAA TCT TTA TAA GTC CTT TT
    • ↵a The numbers in parentheses denote the positions of the initial nucleotides in the primer sequences.

    • ↵b Since the aadA gene sequences for several different organisms are published, the nucleotide number varies and the primers were selected from within a universally conserved sequence.

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Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis
Nancye C. Clark, Ørjan Olsvik, Jana M. Swenson, Carol A. Spiegel, Fred C. Tenover
Antimicrobial Agents and Chemotherapy Jan 1999, 43 (1) 157-160; DOI: 10.1128/AAC.43.1.157

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Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis
Nancye C. Clark, Ørjan Olsvik, Jana M. Swenson, Carol A. Spiegel, Fred C. Tenover
Antimicrobial Agents and Chemotherapy Jan 1999, 43 (1) 157-160; DOI: 10.1128/AAC.43.1.157
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KEYWORDS

Enterococcus faecalis
Nucleotidyltransferases

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