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Mechanisms of Resistance

Characterization of the Chromosomalaac(6′)-Iz Gene of Stenotrophomonas maltophilia

Thierry Lambert, Marie-Cécile Ploy, François Denis, Patrice Courvalin
Thierry Lambert
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,
Centre d’Etudes Pharmaceutiques, 92296 Châtenay-Malabry, and
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Marie-Cécile Ploy
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,
Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 87000 Limoges, France
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François Denis
Laboratoire de Bactériologie-Virologie-Hygiène, CHU Dupuytren, 87000 Limoges, France
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Patrice Courvalin
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,
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DOI: 10.1128/AAC.43.10.2366
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ABSTRACT

The aac(6′)-Iz gene of Stenotrophomonas maltophilia BM2690 encoding an aminoglycoside 6′-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 462 bp corresponding to a protein with a calculated mass of 16,506 Da, a value in good agreement with that of ca. 16,000 found by in vitro coupled transcription-translation. Analysis of the deduced amino acid sequence indicated that the protein was a member of the major subfamily of aminoglycoside 6′-N-acetyltransferases. The enzyme conferred resistance to amikacin but not to gentamicin, indicating that it was an AAC(6′) of type I. The open reading frame upstream from the aac(6′)-Izgene was homologous to the fprA gene of Myxococcus xanthus (61% identity), which encodes a putative pyridoxine (pyridoxamine) 5′-phosphate oxidase. Pulsed-field gel electrophoresis of total DNA from BM2690 and S. maltophilia ATTC 13637 digested with XbaI, DraI, and SpeI followed by hybridization with rRNA and aac(6′)-Iz-specific probes indicated that the gene was located in the chromosome. Theaac(6′)-Iz gene was detected by DNA-DNA hybridization in all 80 strains of S. maltophilia tested. The MICs of gentamicin against these strains of S. maltophilia were lower than those of amikacin, netilmicin, and tobramycin, indicating that production of AAC(6′)-Iz contributes to aminoglycoside resistance in S. maltophilia.

  • Copyright © 1999 American Society for Microbiology
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Characterization of the Chromosomalaac(6′)-Iz Gene of Stenotrophomonas maltophilia
Thierry Lambert, Marie-Cécile Ploy, François Denis, Patrice Courvalin
Antimicrobial Agents and Chemotherapy Oct 1999, 43 (10) 2366-2371; DOI: 10.1128/AAC.43.10.2366

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Characterization of the Chromosomalaac(6′)-Iz Gene of Stenotrophomonas maltophilia
Thierry Lambert, Marie-Cécile Ploy, François Denis, Patrice Courvalin
Antimicrobial Agents and Chemotherapy Oct 1999, 43 (10) 2366-2371; DOI: 10.1128/AAC.43.10.2366
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KEYWORDS

Acetyltransferases
DNA, Bacterial
Xanthomonas

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