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Mechanisms of Resistance

A New Resistance Gene, linB, Conferring Resistance to Lincosamides by Nucleotidylation in Enterococcus faecium HM1025

Bülent Bozdogan, Latifa Berrezouga, Ming-Shang Kuo, David A. Yurek, Kathleen A. Farley, Brian J. Stockman, Roland Leclercq
Bülent Bozdogan
Service de Bactériologie-Virologie, Hôpital Henri Mondor—Université Paris XII, 94000 Créteil, and
Service de Microbiologie, Hôpital Côte de Nacre, Université de Caen, 14033 Caen, France, and
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Latifa Berrezouga
Service de Bactériologie-Virologie, Hôpital Henri Mondor—Université Paris XII, 94000 Créteil, and
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Ming-Shang Kuo
Pharmacia and Upjohn, Kalamazoo, Michigan 49002
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David A. Yurek
Pharmacia and Upjohn, Kalamazoo, Michigan 49002
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Kathleen A. Farley
Pharmacia and Upjohn, Kalamazoo, Michigan 49002
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Brian J. Stockman
Pharmacia and Upjohn, Kalamazoo, Michigan 49002
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Roland Leclercq
Service de Bactériologie-Virologie, Hôpital Henri Mondor—Université Paris XII, 94000 Créteil, and
Service de Microbiologie, Hôpital Côte de Nacre, Université de Caen, 14033 Caen, France, and
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DOI: 10.1128/AAC.43.4.925
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ABSTRACT

Resistance to lincomycin and clindamycin in the clinical isolateEnterococcus faecium HM1025 is due to a ribosomal methylase encoded by an ermAM-like gene and the plasmid-mediated inactivation of these antibiotics. We have cloned and determined the nucleotide sequence of the gene responsible for the inactivation of lincosamides, linB. This gene encodes a 267-amino-acid lincosamide nucleotidyltransferase. The enzyme catalyzes 3-(5′-adenylation) (the adenylation of the hydroxyl group in position 3 of the molecules) of lincomycin and clindamycin. Expression oflinB was observed in both Escherichia coli andStaphylococcus aureus. The deduced amino acid sequence of the enzyme did not display any significant homology with staphylococcal nucleotidyltransferases encoded by linA andlinA′ genes. Sequences homologous to linB were found in 14 other clinical isolates of E. faecium, indicating the spread of the resistance trait in this species.

  • Copyright © 1999 American Society for Microbiology
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A New Resistance Gene, linB, Conferring Resistance to Lincosamides by Nucleotidylation in Enterococcus faecium HM1025
Bülent Bozdogan, Latifa Berrezouga, Ming-Shang Kuo, David A. Yurek, Kathleen A. Farley, Brian J. Stockman, Roland Leclercq
Antimicrobial Agents and Chemotherapy Apr 1999, 43 (4) 925-929; DOI: 10.1128/AAC.43.4.925

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A New Resistance Gene, linB, Conferring Resistance to Lincosamides by Nucleotidylation in Enterococcus faecium HM1025
Bülent Bozdogan, Latifa Berrezouga, Ming-Shang Kuo, David A. Yurek, Kathleen A. Farley, Brian J. Stockman, Roland Leclercq
Antimicrobial Agents and Chemotherapy Apr 1999, 43 (4) 925-929; DOI: 10.1128/AAC.43.4.925
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KEYWORDS

Anti-Bacterial Agents
Enterococcus faecium
Hydrolases
macrolides

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