Article Figures & Data
Figures
- Fig. 1.
Plasmid maps of two resistance constructs with intactarr-2 and disrupted arr-2 and aadB. pCTF104 is a recombinant plasmid created by insertion of aBamHI fragment from the total DNA of PaTh2 into theBamHI site of pUCP24. ori1600 is the origin of replication used by Pseudomonas, rep is the gene coding for replication protein, ColE1 ori is the origin of replication used by E. coli, and aacC1is a gentamicin resistance gene in pUCP24. IS10-like is an insertion element, blaCEF-1 is a CEF-1 β-lactamase gene, aadB is a gentamicin resistance gene,arr-2 is a rifampin resistance gene, and cmlA is a chloramphenicol resistance gene in an insert from PaTh2. pCTF104P is a recombinant plasmid derived from pCTF104.
- Fig. 2.
DNA sequence of the arr-2 cassette and its boundary. The underlined portions are 59-base elements. The double-underlined portions are the core site and the inverse core site of the arr-2 cassette. The boxed codons are the start and stop codons of the arr-2 gene. The vertical bars indicate the boundaries of the cassettes.
- Fig. 3.
CLUSTAL W protein alignment of a putative amino acid sequence of ARR-2 and ARR. The shaded areas indicate identical amino acids, and dashes indicate gaps inserted into the sequence to maximize the alignment.
Tables
- Table 1.
MICs for E. coli and P. aeruginosastrains with various cloned resistance genes
Straina MIC (μg/ml) of: Ampicillin Carbenicillin Ceftazidime Rifampin WTE. coli DH5α 4 4 1 8 DH5α(pCTF104) >2048 >2048 2048 >256 DH5α(pCTF104P) >2048 >2048 2048 8 WT P. aeruginosa PAK 2048 64 8 16 PAK(pCTF104) >2048 >2048 2048 >512 PAK(pCTF104P) >2048 >2048 2048 16 ↵a WT, wild type.
