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Mechanisms of Resistance

Nomenclature for New Tetracycline Resistance Determinants

Stuart B. Levy, Laura M. McMurry, Teresa M. Barbosa, Vickers Burdett, Patrice Courvalin, Wolfgang Hillen, Marilyn C. Roberts, Julian I. Rood, Diane E. Taylor
Stuart B. Levy
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111;
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Laura M. McMurry
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111;
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Teresa M. Barbosa
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111;
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Vickers Burdett
Department of Microbiology and Immunology, Duke University, Durham, North Carolina 27710;
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Patrice Courvalin
Unité des Agents Antibactériens, Institut Pasteur, Paris Cedex 15, France;
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Wolfgang Hillen
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany;
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Marilyn C. Roberts
Department of Pathobiology, University of Washington, Seattle, Washington 98195;
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Julian I. Rood
Department of Microbiology, Monash University, Clayton, Australia; and
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Diane E. Taylor
Departments of Biological Sciences and of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
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DOI: 10.1128/AAC.43.6.1523
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ABSTRACT

Letters of the English alphabet have heretofore been used to name tetracycline resistance determinants. Since all 26 letters have now been used, a nomenclature employing numerals is recommended for future determinants, and one laboratory has offered to coordinate the assignment of numerals.

In 1989, a Note describing the nomenclature for tetracycline resistance determinants which employed letters of the English alphabet was published in this journal (14). Since no letters now remain for designating additional determinants, we propose that new determinants be hereafter designated by Arabic numerals. The new system is patterned on the previous letter-based one, with a numeral used instead of a letter to name a determinant. Since 30 determinants have been described (most, but not all, according to the 1989 nomenclature, with the new 1999 nomenclature being used for Tet 30) (Table 1), the next determinant would receive number 31, with no renaming of the earlier determinants. Following the previous system used with letters, the class would be 31, and the determinant would be designated Tet 31 (with a space between “Tet” and “31”). If there were only a single gene in the determinant, it would be designatedtet(31). If there were more than one structural gene, the first would be designated tetA(31) and the second would betetB(31), etc. A regulatory gene would be designatedtetR(31). Note that the class designation, 31, is not italicized. The names of the corresponding proteins would be Tet(31) or TetA(31), etc. An allele of a gene would be designated by a hyphen followed by an italicized allele number; for example,tetA(31)-1 would be allele number 1 of thetetA gene of class 31. If the class designation is not needed within a single communication, it could be omitted.

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Table 1.

Known tetracycline resistance determinantsa

This system employs some of the conventions from the previous communication (14) and is summarized in Table2. Usage for previously described determinants should continue to conform to the prior recommendations (14).

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Table 2.

Proposed nomenclature for new tetracycline resistance determinants

Recently, there have been several situations in which tetracycline resistance determinants discovered in different laboratories were nearly given the same designation. To avoid such a problem in the future, we offer the S. B. Levy group to coordinate the naming of new determinants. Such a determinant can be defined as a naturally occurring unit of one or more adjacent genes involved primarily in tetracycline resistance (as opposed to multidrug resistance or other known function) having a sequence significantly different from sequences of currently known determinants. We suggest ≤80% amino acid identity as the dividing line, based on previous usage. Class L has been defined as a single class even though it contains two subgroups in which the single proteins only are 81% identical (25, 26), while the proteins of two different classes, M and S, are 79% identical (4, 25). To confirm that a number proposed for a newly discovered tetracycline resistance determinant has not been used, please contact S. B. Levy.

Finally, we note that when looking in databases for a name containing parentheses or a space, such as “tet(X)” or “Tet X”, quotation marks should be used around the name, as shown, to retrieve the term intact.

ACKNOWLEDGMENTS

This work was supported by grant GM55430 from the National Institutes of Health.

FOOTNOTES

    • Received 24 February 1999.
    • Accepted 24 March 1999.
  • Copyright © 1999 American Society for Microbiology

REFERENCES

  1. 1.
    1. Allard J. D.,
    2. Bertrand K. P.
    Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins. J. Bacteriol. 175 1993 4554 4560
    OpenUrlAbstract/FREE Full Text
  2. 2.
    1. Allard J. D.,
    2. Gibson M. L.,
    3. Vu L. H.,
    4. Nguyen T. T.,
    5. Bertrand K. P.
    Nucleotide sequence of class D tetracycline resistance genes from Salmonella ordonez. Mol. Gen. Genet. 237 1993 301 305
    OpenUrlCrossRefPubMed
  3. 3.
    1. Barbosa T. M.,
    2. Scott K. P.,
    3. Flint H. J.
    Evidence for recent intergenic transfer of a new tetracycline resistance gene, tet(W), isolated from Butyrivibrio fibrisolvens, and the occurrence of tet(O), in ruminal bacteria. Environ. Microbiol. 1 1999 53 64
    OpenUrlCrossRefPubMedWeb of Science
  4. 4.↵
    1. Charpentier E.,
    2. Gerbaud G.,
    3. Courvalin P.
    Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210. Gene 131 1993 27 34
    OpenUrlCrossRefPubMedWeb of Science
  5. 5.
    1. Clermont D.,
    2. Chesneau O.,
    3. de Cespédès G.,
    4. Horaud T.
    New tetracycline resistance determinants coding for ribosomal protection in streptococci and nucleotide sequence of tet(T) isolated from Streptococcus pyogenes A498. Antimicrob. Agents Chemother. 41 1997 112 116
    OpenUrlAbstract/FREE Full Text
  6. 6.
    1. Dairi T.,
    2. Aisaka K.,
    3. Katsumata R.,
    4. Hasegawa M.
    A self-defense gene homologous to tetracycline effluxing gene essential for antibiotic production in Streptomyces aureofaciens. Biosci. Biotechnol. Biochem. 59 1995 1835 1841
    OpenUrlCrossRefPubMedWeb of Science
  7. 7.
    1. de Rossi E.,
    2. Blokpoel M. C. J.,
    3. Cantoni R.,
    4. Branzoni M.,
    5. Riccardi G.,
    6. Young D. B.,
    7. De Smet K. A. L.,
    8. Ciferri O.
    Molecular cloning and functional analysis of a novel tetracycline resistance determinant, tet(V), from Mycobacterium smegmatis. Antimicrob. Agents Chemother. 42 1998 1931 1937
    OpenUrlAbstract/FREE Full Text
  8. 8.
    1. Dittrich W.,
    2. Schrempf H.
    The unstable tetracycline resistance gene of Streptomyces lividans 1326 encodes a putative protein with similarities to translational elongation factors and Tet(M) and Tet(O) proteins. Antimicrob. Agents Chemother. 36 1992 1119 1124
    OpenUrlAbstract/FREE Full Text
  9. 9.
    1. Doyle D.,
    2. McDowall K. J.,
    3. Butler M. J.,
    4. Hunter I. S.
    Characterization of an oxytetracycline-resistance gene, otrA, of Streptomyces rimosus. Mol. Microbiol. 5 1991 2923 2933
    OpenUrlCrossRefPubMedWeb of Science
  10. 10.
    1. Hansen L. M.,
    2. McMurry L. M.,
    3. Levy S. B.,
    4. Hirsh D. C.
    A new tetracycline resistance determinant, Tet H, from Pasteurella multocida specifying active efflux of tetracycline. Antimicrob. Agents Chemother. 37 1993 2699 2705
    OpenUrlAbstract/FREE Full Text
  11. 11.
    1. Hillen W.,
    2. Shollmeier K.
    Nucleotide sequence of the Tn10 encoded tetracycline resistance gene. Nucleic Acids Res. 11 1983 525 539
    OpenUrlCrossRefPubMedWeb of Science
  12. 12.
    1. Horaud T.,
    2. Delbos F.,
    3. Pepper K.
    Does a tetracycline resistance determinant of class N exist? Antimicrob. Agents Chemother. 34 1990 1447 1449
    OpenUrlAbstract/FREE Full Text
  13. 13.
    1. Hoshino T.,
    2. Ikeda T.,
    3. Tomizuka N.,
    4. Furukawa K.
    Nucleotide sequence of the tetracycline resistance gene of pTHT15, a thermophilic Bacillis plasmid: comparison with staphylococcal TcR controls. Gene 37 1985 131 138
    OpenUrlCrossRefPubMedWeb of Science
  14. 14.↵
    1. Levy S. B.,
    2. McMurry L. M.,
    3. Burdett V.,
    4. Courvalin P.,
    5. Hillen W.,
    6. Roberts M. C.,
    7. Taylor D. E.
    Nomenclature for tetracycline resistance determinants. Antimicrob. Agents Chemother. 33 1989 1373 1374
    OpenUrlAbstract/FREE Full Text
  15. 15.
    1. Luo Z.-Q.,
    2. Farrand S. K.
    Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58. J. Bacteriol. 181 1999 618 626
    OpenUrlAbstract/FREE Full Text
  16. 16.
    1. Magalhaes V. D.,
    2. Schuman W.,
    3. Castilho B. A.
    A new tetracycline resistance determinant cloned from Proteus mirabilis. Biochim. Biophys. Acta 1443 1998 262 266
    OpenUrlPubMed
  17. 17.
    1. Martin P.,
    2. Trieu-Cuot P.,
    3. Courvalin P.
    Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative transposon Tn 1545 . Nucleic Acids Res. 14 1986 7047 7058
    OpenUrlCrossRefPubMedWeb of Science
  18. 18.
    1. McMurry L. M.,
    2. Levy S. B.
    Revised sequence of OtrB (Tet347) tetracycline efflux protein from Streptomyces rimosus. Antimicrob. Agents Chemother. 42 1998 3050
    OpenUrlFREE Full Text
  19. 19.
    1. Nikolich M. P.,
    2. Shoemaker N. B.,
    3. Salyers A. A.
    A Bacteroides tetracycline resistance gene represents a new class of ribosome protection tetracycline resistance. Antimicrob. Agent Chemother. 36 1992 1005 1012
    OpenUrlAbstract/FREE Full Text
  20. 20.
    1. Noguchi N.,
    2. Aoki T.,
    3. Sasatsu M.,
    4. Kono M.,
    5. Shishido K.,
    6. Ando T.
    Determination of the complete nucleotide sequence of pNS12, a staphylococcal tetracycline-resistance plasmid propagated in Bacillus subtilis. FEMS Microbiol. Lett. 37 1986 283 288
    OpenUrlCrossRefWeb of Science
  21. 21.
    1. Pang Y.,
    2. Brown B. A.,
    3. Steingrube V. A.,
    4. Wallace R. J. Jr.,
    5. Roberts M. C.
    Tetracycline resistance determinants in Mycobacterium and Streptomyces species. Antimicrob. Agents Chemother. 38 1994 1408 1412
    OpenUrlAbstract/FREE Full Text
  22. 22.
    1. Park B. H.,
    2. Hendricks M.,
    3. Malamy M. H.,
    4. Tally F. P.,
    5. Levy S. B.
    Cryptic tetracycline resistance determinant (class F) from Bacteroides fragilis mediates resistance in Escherichia coli by actively reducing tetracycline accumulation. Antimicrob. Agents Chemother. 31 1987 1739 1743
    OpenUrlAbstract/FREE Full Text
  23. 23.
    1. Peden K. W. C.
    Revised sequence of the tetracycline resistance gene of pBR322. Gene 22 1983 277 280
    OpenUrlCrossRefPubMedWeb of Science
  24. 24.
    1. Ridenhour M. B.,
    2. Fletcher H. M.,
    3. Mortensen J. E.,
    4. Daneo-Moore L.
    A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKQ10 in Enterococcus faecium. Plasmid 35 1996 71 80
    OpenUrlCrossRefPubMed
  25. 25.↵
    1. Roberts M. C.
    Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution. FEMS Microbiol. Rev. 19 1996 1 24
    OpenUrlCrossRefPubMed
  26. 26.↵
    1. Sakaguchi R.,
    2. Amano H.,
    3. Shishido K.
    Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981. Biochim. Biophys. Acta 950 1988 441 444
    OpenUrlCrossRefPubMedWeb of Science
  27. 27.
    1. Sloan J.,
    2. McMurry L. M.,
    3. Lyras D.,
    4. Levy S. B.,
    5. Rood J. I.
    The Clostridium perfringens Tet P determinant comprises two overlapping genes: tetA(P), which mediates active tetracycline efflux, and tetB(P), which is related to the ribosomal protection family of tetracycline-resistance determinants. Mol. Microbiol. 11 1994 403 415
    OpenUrlCrossRefPubMed
  28. 28.
    1. Sougakoff W.,
    2. Papadopoulou B.,
    3. Nordmann P.,
    4. Courvalin P.
    Nucleotide sequence and distribution of gene tetO encoding tetracycline resistance in Campylobacter coli. FEMS Microbiol. Lett. 44 1987 153 159
    OpenUrlCrossRefWeb of Science
  29. 29.
    1. Speer B. S.,
    2. Bedzyk L.,
    3. Salyers A. A.
    Evidence that a novel tetracycline resistance gene found on two Bacteroides transposons encodes an NADP-requiring oxidoreductase. J. Bacteriol. 173 1991 176 183
    OpenUrlAbstract/FREE Full Text
  30. 30.
    1. Speer B. S.,
    2. Salyers A. A.
    A tetracycline efflux gene on Bacteroides transposon Tn4400 does not contribute to tetracycline resistance. J. Bacteriol. 172 1990 292 298
    OpenUrlAbstract/FREE Full Text
  31. 31.
    Tauch, A., A. Puehler, and J. Kalinowski. DNA sequence and genetic organization of pAG1, a 19.8-kb R-plasmid ofCorynebacterium glutamicum encoding a new class of tetracycline efflux and repressor proteins. Submitted for publication. Unpublished data (GenBank accession no. AF121000).
  32. 32.
    Tietze, E. 1998. Unpublished data (in GenBank).
  33. 33.
    1. Waters S. H.,
    2. Rogowsky P.,
    3. Grinsted J.,
    4. Altenbuchner J.,
    5. Schmitt R.
    The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis. Nucleic Acids Res. 11 1983 6089 6105
    OpenUrlCrossRefPubMedWeb of Science
  34. 34.
    1. Zhao J.,
    2. Aoki T.
    Nucleotide sequence analysis of the class G tetracycline resistance determinant from Vibrio anguillarum. Microbiol. Immunol. 36 1992 1051 1060
    OpenUrlCrossRefPubMedWeb of Science
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Nomenclature for New Tetracycline Resistance Determinants
Stuart B. Levy, Laura M. McMurry, Teresa M. Barbosa, Vickers Burdett, Patrice Courvalin, Wolfgang Hillen, Marilyn C. Roberts, Julian I. Rood, Diane E. Taylor
Antimicrobial Agents and Chemotherapy Jun 1999, 43 (6) 1523-1524; DOI: 10.1128/AAC.43.6.1523

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Nomenclature for New Tetracycline Resistance Determinants
Stuart B. Levy, Laura M. McMurry, Teresa M. Barbosa, Vickers Burdett, Patrice Courvalin, Wolfgang Hillen, Marilyn C. Roberts, Julian I. Rood, Diane E. Taylor
Antimicrobial Agents and Chemotherapy Jun 1999, 43 (6) 1523-1524; DOI: 10.1128/AAC.43.6.1523
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KEYWORDS

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Tetracycline Resistance

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