Activities of Sordarins in Murine Histoplasmosis

DOI: 10.1128/AAC.43.7.1716

Article Figures & Data

Table 2.

Survival of mice infected intravenously withH. capsulatum^a

Study and inoculum (CFU)	Drug or treatment	Dose (mg/kg)	No. of days of survival		No. alive at day 30
Study and inoculum (CFU)	Drug or treatment	Dose (mg/kg)	Mean	SEM	No. alive at day 30
Study A (2.0 × 10⁶)	Control	None	15.2	2.9	2
	GM2	20	30^b	0	10
		10	28.4^b	2.6	9
		5	29.1^b	1.9	9
		2	30^b	0	10
	Amphotericin B	1	30^b	0	10
		0.5	30^b	0	10
	Fluconazole	10	23.2	4.0	7
		5	20.1	3.7	5
Study B (10⁸)	Control	None	4.0	0.14	0
	GM3	5	6.7^b	0.9	0
		1.25	4.9	0.4	0
		0.3	4.0	0.3	0
		0.06	4.1	0.2	0
	Amphotericin B	1.25	28.4^b	2.6	9
		0.3	4.8	0.2	0
	Fluconazole	10	13.1^b	3.2	2

↵a Mice were treated from day 2 through 8 after infection, and their rates of survival were observed through day 30. Ten mice were in each treatment group.
↵b P < 0.001 compared with the control group.

Table 3.

Lung and kidney tissue burdens^a

Tissue	Drug or treatment	Dose (mg/kg)	Mean burden	95% CI		No. of mice with counts of <20 CFU/g
Tissue	Drug or treatment	Dose (mg/kg)	Mean burden	Lower	Upper	No. of mice with counts of <20 CFU/g
Kidney	Control	None	2.3 × 10⁷	1.2 × 10⁷	5.7 × 10⁷	0
	GM3	10	4.8 × 10^{4 b}	2.5 × 10⁴	1.2 × 10⁵	0
		5	8.0 × 10^{5 b}	4.3 × 10⁵	2.0 × 10⁶	0
		1.25	1.3 × 10⁸	6.9 × 10⁷	3.2 × 10⁸	0
	Amphotericin B	2.5	2.3 × 10^{2 c}	1.2 × 10²	5.7 × 10²	3
	Fluconazole	5	3.4 × 10^{6 b}	1.8 × 10⁶	8.3 × 10⁶	0
Lung	Control	None	5.9 × 10⁷	3.1 × 10⁷	1.6 × 10⁸	1
	GM3	10	1.1 × 10^{5 b}	5.7 × 10⁴	2.6 × 10⁵	0
		5	2.9 × 10^{6 b}	1.5 × 10⁶	7.8 × 10⁶	1
		1.25	1.9 × 10⁸	1.0 × 10⁸	4.8 × 10⁸	0
	Amphotericin B	2.5	1.2 × 10^{2 c}	6.7 × 10¹	3.1 × 10²	6
	Fluconazole	5	1.3 × 10^{7 b}	6.8 × 10⁶	3.1 × 10⁷	0

↵a Values are expressed as CFU per gram of tissue. Each treatment group consisted of seven mice. CI, confidence interval.
b P < 0.05 compared with control group.
c P < 0.01 compared with control group or groups receiving fluconazole or GM3.