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Mechanisms of Resistance

Production of a TEM-24 Plasmid-Mediated Extended-Spectrum β-Lactamase by a Clinical Isolate ofPseudomonas aeruginosa

Helene Marchandin, Helene Jean-Pierre, Christophe De Champs, Danielle Sirot, Helene Darbas, Pierre Francois Perigault, Christian Carriere
Helene Marchandin
Service de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5,
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Helene Jean-Pierre
Service de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5,
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Christophe De Champs
Service de Bactériologie, Faculté de Médecine et de Pharmacie, 63001 Clermont Ferrand, and
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Danielle Sirot
Service de Bactériologie, Faculté de Médecine et de Pharmacie, 63001 Clermont Ferrand, and
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Helene Darbas
Service de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5,
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Pierre Francois Perigault
Département d'Anesthésie et de Réanimation DARB, Hôpital Saint Eloi, 34000 Montpellier, France
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Christian Carriere
Service de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5,
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DOI: 10.1128/AAC.44.1.213-216.2000
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    Fig. 1.

    PFGE of total XbaI-restricted DNA from ESBL-producing E. aerogenes strains. M, lambda ladder. ESBL-producing E. aerogenes strains were isolated from a patient's postsurgical wound (lanes 1 and 2), feces (lanes 3 and 4), and urine (lane 5). ESBL-producing E. aerogenes strains were isolated from three other patients hospitalized in three different ICUs in our hospital (lanes 6 to 8). The size of the ladder is indicated in kilobases.

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    Fig. 2.

    PFGE of total SpeI-restricted DNA fromP. aeruginosa strains. M, lambda ladder. P. aeruginosa strains were isolated from the same patient's wound (lane 1), urine (lanes 4 and 6, ESBL-producing P. aeruginosa; lane 7, β-lactam susceptible P. aeruginosa), and sputum (lane 2, 3, and 5). The sizes are indicated in kilobases.

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  • Table 1.

    Isolates, sites of infection and antimicrobial treatmentsa

    Embedded Image
    • ↵a ISP, isepamicin; CS, colistin; FT, nitrofurantoin; S, β-lactam susceptible; IPM R, imipenem resistant; CAZ R, ceftazidime resistant; EAER: E. aerogenes; ECOL;E. coli; PMIR, P. mirabilis; PAO3, P. aeruginosa O:3; PAO4, P. aeruginosa O:4.

  • Table 2.

    Antimicrobial susceptibilities of multiresistant clinical isolates and their transconjugants in E. coli HB101

    StrainaMIC (μg/ml) ofb:
    TICPIPCTXCAZFEPATMIPM
    PAO3S328162442
    PAO4 CAZ R, IPM R64128>12832321664
    PAO3ESBL>1281664>12832322
    TrPAO3ESBL>128160.5640.254ND
    E. aerogenes>128648>1281161
    TrE. aerogenes>128160.5640.254ND
    E. coli>128160.51280.540.5
    Tr E. coli>128160.5640.254ND
    P. mirabilis>128160.1280.50.121
    TrP. mirabilis>128160.5640.254ND
    • ↵a Tr, transconjugant in E. coli HB101; PAO3 S, first susceptible P. aeruginosa strain isolated from wound; PAO4 CAZ R, IPM R, multidrug-resistant P. aeruginosa from sputum;PAO3 ESBL, ESBL-producing P. aeruginosa from urinary tract.

    • ↵b TIC, ticarcillin; PIP, piperacillin; CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; ATM, aztreonam; IPM, imipenem; ND, not determined.

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Production of a TEM-24 Plasmid-Mediated Extended-Spectrum β-Lactamase by a Clinical Isolate ofPseudomonas aeruginosa
Helene Marchandin, Helene Jean-Pierre, Christophe De Champs, Danielle Sirot, Helene Darbas, Pierre Francois Perigault, Christian Carriere
Antimicrobial Agents and Chemotherapy Jan 2000, 44 (1) 213-216; DOI: 10.1128/AAC.44.1.213-216.2000

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Production of a TEM-24 Plasmid-Mediated Extended-Spectrum β-Lactamase by a Clinical Isolate ofPseudomonas aeruginosa
Helene Marchandin, Helene Jean-Pierre, Christophe De Champs, Danielle Sirot, Helene Darbas, Pierre Francois Perigault, Christian Carriere
Antimicrobial Agents and Chemotherapy Jan 2000, 44 (1) 213-216; DOI: 10.1128/AAC.44.1.213-216.2000
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KEYWORDS

plasmids
Pseudomonas aeruginosa
beta-lactamases

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