Susceptibilities of Oral and Nasal Isolates ofStreptococcus mitis and Streptococcus oralis to Macrolides and PCR Detection of Resistance Genes

Viridans group streptococci, commensal bacteria in the human oral and nasal cavities, are associated with systemic diseases, including infective endocarditis, bacteremia, and pneumonia (2, 5, 6, 10, 12). Macrolide resistance has spread in staphylococci, enterococci, and streptococci (7-9, 11), but little is known about the distribution of the resistance among oral streptococci (12, 19). In this work, clinical isolates of oral and nasal Streptococcus mitis andStreptococcus oralis have been tested for susceptibility to macrolides, and resistance genes have been characterized.

Eighty-four streptococcal isolates from patients who visited Tokushima University Hospital between June and December 1995 were studied. These strains were isolated from periodontal pockets, larynx, pharynx, maxillary sinus, and nasal secretion and identified as S. oralis (54 strains), S. mitis (29 strains),Streptococcus sanguis (1 strain), and Streptococcus salivarius (1 strain) by biochemical tests, including the API-20 Strep system (bioMérieux, La Balme des Grottes, France).

The following antibiotics were used: leucomycin and midecamycin (Meiji Pharmaceutical Co., Ltd., Tokyo, Japan), erythromycin and rokitamycin (Asahi Kasei Co., Ltd., Tokyo, Japan), clarithromycin (Dynabott), azithromycin (Pfizer Pharmaceuticals), and roxithromycin (Hoechst-Marion-Roussel).

MICs were determined by a broth microdilution method in anaerobic MIC broth (Difco, Detroit, Mich.). Microtiter plates were incubated at 37°C for 24 h in 5% CO₂.

Induction experiments for macrolide resistance were performed by preculture with a sub-MIC of erythromycin. Crude DNA of streptococci was prepared as previously described (13). PCR primers forerm and mefE were designed from published sequences (1) to provide specific PCR products of 530 and 1,218 bp, respectively. The erm primers were 5′-GAAATIGGIIIIGGIAAAGGICA-3′ and 5′-AAYTGRTTYTTIGTRAA-3′, and the mefE primers were 5′-ATGGAAAAATACAACAATTGGAAACGA-3′ and 5′-TTATTTTAAATCTAATTTTCTAACCTC-3′.

Macrolide susceptibility is shown in Table1. The erythromycin MICs at which 90% of the isolates tested are inhibited (MIC₉₀s) for S. oralis and S. mitis were 8 and 32 μg/ml, respectively. MIC ranges of clarithromycin, azithromycin, and roxithromycin were similar to those of erythromycin. The MIC range of rokitamycin for S. oralis and S. mitis was narrow, with MIC₉₀s of 0.5 and 1 μg/ml, respectively. Among 13 erythromycin-resistant strains (MIC, ≥8 μg/ml), 6 strains were highly resistant to erythromycin; the MICs for them were ≥512 μg/ml (Table 2). All of the strains except O14 were also highly resistant to clarithromycin, azithromycin, and roxithromycin, but intermediately resistant to rokitamycin (MIC, ≥0.5 to ≤2 μg/ml). Strain O14 was intermediately resistant to azithromycin and roxithromycin (MICs, ≥0.5 to ≤2 μg/ml) and sensitive to rokitamycin (MIC, ≤0.25 μg/ml). Seven strains were intermediately resistant to 14- and 15-member macrolides.

As shown in Table 2, all erythromycin-resistant strains were more sensitive to rokitamycin than other macrolides. To determine whether resistance could be induced, MICs of 16-member macrolides for cells grown in medium with or without a sub-MIC of erythromycin were examined. Highly resistant strains SO12, SO13, O14, E2, and E21 were induced to develop resistance to rokitamycin, midecamycin, and leucomycin. Strain E3 was highly resistant to midecamycin and leucomycin, even when cultured without erythromycin, and rokitamycin resistance was not induced. The intermediately resistant strains, O24, E17, E11, E27, and E30, did not develop resistance to 16-member macrolides after erythromycin induction.

To clarify the mechanisms of resistance, PCR amplification of macrolide-resistant genes was performed. PCR primers for theerm (23S rRNA methylase) gene were designed based on the sequence of corresponding genes from other organisms. The highly resistant strains gave a distinct band of the expected 530-bp size from position 103 to position 633 of the erm gene. PCR products from these strains and S. salivarius E37 were sequenced (Fig. 1). Sequences from strains E2, E21, and E30 were identical to those of the gene encoding the rRNA methylase on transposon Tn917 (4). Those from other strains had ∼98% homology to Tn917. The nucleotide changes in strains SO12, SO13, and E37 resulted in six, four, and two amino acid alterations, respectively, but did not affect the reading frame. Primer sets for the mefE gene were designed based on the sequence of that gene in S. pneumoniae (GenBank U83667). The intermediately resistant strains O24, E17, and E3 and highly resistant strain O14 gave the expected band (approximately 1,200 bp) for themefE gene encoding the macrolide efflux pump (18). The sequences of DNA obtained from PCR amplification for mefE in strains O24, E3, and E17 were analyzed. These sequences were identical to those at positions 30 to 1190 of the corresponding mefE gene. Less erythromycin accumulated inmefE-positive strain E17 than in mefE-negative strain E1 15 min after the addition of [N-methyl-¹⁴C]erythromycin (data not shown). Accumulation of erythromycin in the mefE-positive strain was restored by addition of proton conductors, suggesting that a macrolide efflux system exists in strain E17. For genes encoding macrolide-inactivating enzyme, ereA, ereB, andmphE and macrolide efflux genes msr andmefA (17), PCR amplification was performed with DNA from macrolide-resistant strains; however, no PCR products have been detected.

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Fig. 1.

Nucleotide sequences of PCR products obtained with oral streptococci by using an erm primer set. The sequences in strains E2, E21, and E30 were identical to that from the site of theE. faecalis ermB gene. In strains E37, SO12, and SO13, only the base changes are shown (highlighted by asterisks).

Although erm genes encoding rRNA methylase are present in various organisms, such as Escherichia coli, Bacillus subtilis, and Staphylococcus aureus, and macrolide resistance is widespread in bacteria associated with humans (4, 15, 17), the potential reservoir for erm genes is unknown. It has been reported that the genes lie on various transposable elements or conjugal plasmids (4, 11, 14). In the present study, we showed that the nucleotide sequences of PCR products obtained with erm primers from some strains were identical to the rRNA methylase gene in Enterococcus faecalis transposon Tn917, while those from S. oralis SO12 and S. mitis SO13 were highly homologous with the ermB gene from conjugal plasmid pIP501 ofStreptococcus agalactiae (3, 4). In the upstream region, the sequence homologous to LR, an internal sequence of Tn917 has been found (16) (data not shown).S. oralis and S. mitis are major species in the oral and nasal normal flora. The results of this study suggest the transmission of macrolide-resistant genes between oral streptococci and other more virulent streptococci.

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