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Mechanisms of Resistance

Clinical Isolate of Vancomycin-HeterointermediateStaphylococcus aureus Susceptible to Methicillin and In Vitro Selection of a Vancomycin-Resistant Derivative

Sophie Bobin-Dubreux, Marie-Elisabeth Reverdy, Chantal Nervi, Martine Rougier, Anne Bolmström, François Vandenesch, Jerome Etienne
Sophie Bobin-Dubreux
Laboratoire de Microbiologie, EA 1655, Hôpital Edouard Herriot, 69437 Lyon cedex 03, France, and
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Marie-Elisabeth Reverdy
Laboratoire de Microbiologie, EA 1655, Hôpital Edouard Herriot, 69437 Lyon cedex 03, France, and
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Chantal Nervi
Laboratoire de Microbiologie, EA 1655, Hôpital Edouard Herriot, 69437 Lyon cedex 03, France, and
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Martine Rougier
Laboratoire de Microbiologie, EA 1655, Hôpital Edouard Herriot, 69437 Lyon cedex 03, France, and
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Anne Bolmström
AB BIODISK, S-169 56 Solna, Sweden
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François Vandenesch
Laboratoire de Microbiologie, EA 1655, Hôpital Edouard Herriot, 69437 Lyon cedex 03, France, and
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Jerome Etienne
Laboratoire de Microbiologie, EA 1655, Hôpital Edouard Herriot, 69437 Lyon cedex 03, France, and
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DOI: 10.1128/AAC.45.1.349-352.2001
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    Fig. 1.

    Population analysis of parental hVISA strain MER,S. aureus ATCC 29213, and their derivatives. Bacteria grown overnight were serially diluted and plated on various concentrations of vancomycin-containing agar medium. MER-S6 was a MER-derived progeny selected at 6 μg of vancomycin per ml, MER-S12 was derived from MER-S6 selected at 12 μg of vancomycin per ml, and MER-S20 was derived from MER-S12 selected at 20 μg of vancomycin per ml. ATCC 29213-S3 was selected at 3 μg of vancomycin per ml, ATCC 29213-S4 was selected at 4 μg of vancomycin per ml, and ATCC 29213-S4′ was obtained by selecting ATCC 29213-S4 with 4 μg of vancomycin per ml. Horizontal arrows indicate the order in which the consecutive population analyses were performed.

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    Fig. 2.

    Composite picture showing the morphology of S. aureus ATCC 29213 (A) susceptible to vancomycin and the morphological abnormalities of hVISA MER grown in the absence (B) or presence (C) of 2 μg of vancomycin per ml and of strain MER-S20 (D). All cultures were grown to an optical density at 600 nm of 0.6 in brain heart infusion broth prior to processing for transmission electron microscopic examination. Magnification, ×40,000 (original magnification, ×60,000).

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    MICs of vancomycin and teicoplanin for hVISA, VISA, andS. aureus ATCC 29213

    StrainMIC (μg/ml) determined by:
    Etest witha:
    NCCLS broth microdilution0.5-McFarland inoculum (MHA)2-McFarland inoculum (BHI)
    VancomycinTeicoplaninVancomycinTeicoplaninVancomycinTeicoplanin
    MER4444612
    MER-S6884241248
    MER-S128164321648
    MER-S208168322464
    Mu32828416
    Mu5048481224
    LIM-12121.544
    LIM-24821.5824
    ATCC 292130.50.510.7522
    • ↵a MHA, Mueller-Hinton agar plate; BHI, brain heart infusion plate.

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Clinical Isolate of Vancomycin-HeterointermediateStaphylococcus aureus Susceptible to Methicillin and In Vitro Selection of a Vancomycin-Resistant Derivative
Sophie Bobin-Dubreux, Marie-Elisabeth Reverdy, Chantal Nervi, Martine Rougier, Anne Bolmström, François Vandenesch, Jerome Etienne
Antimicrobial Agents and Chemotherapy Jan 2001, 45 (1) 349-352; DOI: 10.1128/AAC.45.1.349-352.2001

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Clinical Isolate of Vancomycin-HeterointermediateStaphylococcus aureus Susceptible to Methicillin and In Vitro Selection of a Vancomycin-Resistant Derivative
Sophie Bobin-Dubreux, Marie-Elisabeth Reverdy, Chantal Nervi, Martine Rougier, Anne Bolmström, François Vandenesch, Jerome Etienne
Antimicrobial Agents and Chemotherapy Jan 2001, 45 (1) 349-352; DOI: 10.1128/AAC.45.1.349-352.2001
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KEYWORDS

Anti-Bacterial Agents
Methicillin
penicillins
Staphylococcus aureus
vancomycin

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