Article Figures & Data
Figures
- Fig. 1.
Population analysis of parental hVISA strain MER,S. aureus ATCC 29213, and their derivatives. Bacteria grown overnight were serially diluted and plated on various concentrations of vancomycin-containing agar medium. MER-S6 was a MER-derived progeny selected at 6 μg of vancomycin per ml, MER-S12 was derived from MER-S6 selected at 12 μg of vancomycin per ml, and MER-S20 was derived from MER-S12 selected at 20 μg of vancomycin per ml. ATCC 29213-S3 was selected at 3 μg of vancomycin per ml, ATCC 29213-S4 was selected at 4 μg of vancomycin per ml, and ATCC 29213-S4′ was obtained by selecting ATCC 29213-S4 with 4 μg of vancomycin per ml. Horizontal arrows indicate the order in which the consecutive population analyses were performed.
- Fig. 2.
Composite picture showing the morphology of S. aureus ATCC 29213 (A) susceptible to vancomycin and the morphological abnormalities of hVISA MER grown in the absence (B) or presence (C) of 2 μg of vancomycin per ml and of strain MER-S20 (D). All cultures were grown to an optical density at 600 nm of 0.6 in brain heart infusion broth prior to processing for transmission electron microscopic examination. Magnification, ×40,000 (original magnification, ×60,000).
Tables
- Table 1.
MICs of vancomycin and teicoplanin for hVISA, VISA, andS. aureus ATCC 29213
Strain MIC (μg/ml) determined by: Etest witha: NCCLS broth microdilution 0.5-McFarland inoculum (MHA) 2-McFarland inoculum (BHI) Vancomycin Teicoplanin Vancomycin Teicoplanin Vancomycin Teicoplanin MER 4 4 4 4 6 12 MER-S6 8 8 4 24 12 48 MER-S12 8 16 4 32 16 48 MER-S20 8 16 8 32 24 64 Mu3 2 8 2 8 4 16 Mu50 4 8 4 8 12 24 LIM-1 2 1 2 1.5 4 4 LIM-2 4 8 2 1.5 8 24 ATCC 29213 0.5 0.5 1 0.75 2 2 ↵a MHA, Mueller-Hinton agar plate; BHI, brain heart infusion plate.
