DOI: 10.1128/AAC.45.12.3657-3659.2001
Article Figures & Data
Figures
- Fig. 1.
Effect of DPM on HSV replication. HSV KOS at a multiplicity of infection of 0.01 was added to confluent Vero cell monolayers in 35-mm-diameter plates. After adsorption for 1 h at 37°C, medium with or without DPM was added, and the plates were incubated at 37°C. Plates were freeze-thawed at the times indicated, and supernatant fluids were titrated on Vero cell monolayers. The results are the average of two independent studies.
Tables
- Table 1.
TdR-enhanced reactivation of TK-negative HSV (dlsactk) and DPM inhibition of this effect
Supplemental TdR in medium (concn) No. of ganglia reactivated/no. tested (% positive) in medium with DPM at indicated concn (μM) 0 25 50 No 0/10 (0) NDa ND Yes (100 μM) 10/10 (100) 10/18 (56) 0/19 (0) ↵a ND, not done.
- Table 2.
DPM inhibition of wild-type HSV (strain KOS) reactivation
Tissue tested No. of ganglia reactivated/no. tested (% positive) in medium with DPM at indicated concn (μM) 0 25 50 DRG 10/10 (100) 1/12 (8) 0/12 (0) TG 10/10 (100) 6/12 (50) 1/12 (8) - Table 3.
Decrease of the DPM inhibitory effect on wild-type HSV (strain KOS) reactivation by TdRa
Tissue tested No. of ganglia reactivated/no. tested (% positive) in medium with TdR at indicated concn (μM) 0 50 100 200 DRG 0/10 (0) 4/11 (36) 6/11 (54) 6/11 (54) TG 3/10 (30) 6/11 (54) 8/11 (73) 7/11 (64) ↵a DPM was added to the medium at a concentration of 25 μM.
