Article Figures & Data
Figures
- Fig. 1.
Nucleotide sequence of 2,254 bp of recombinant plasmid pDG-1 containing the bla OXA-28 coding region and part of its integron, In57. The deduced amino acid sequence is designated in single-letter code below the nucleotide sequence. The slash indicates the putative clevage site for the leader peptide for the mature OXA-28 β-lactamase. Underlined amino acid sequences are those conserved for the oxacillinase family and are numbered according to DBL numbering (3). The start codons ofintI1, bla OXA-28, andaac(6′)Ib are indicated by horizontal arrows, and their stop codons are indicated by asterisks. The −35 and −10 sequences of the promoters Pc, P2, and Pint are underlined. The conserved core and inverse core sites located at theoxa28 and aac(6′)Ib cassette boundaries are boxed and the composite 59-base elements are italicized. The additional base of the 59-base element of the oxa28 gene cassette as compared to the 59-base element of the oxa19 gene cassette is indicated by a square below the 59-base element of theoxa28 gene cassette. The cassette boundaries are indicated by vertical arrows. The left part of the attI1 site is underlined with a dotted line.
Tables
- Table 1.
MICs of β-lactams for the P. aeruginosa ED-1 clinical isolate, its transconjugant P. aeruginosaPU21(pGIR-1), reference strain P. aeruginosa PU21,E. coli DH10B(pDG-1), and reference strain E. coli DH10B
β-Lactama MIC (μg/ml) for: P. aeruginosa ED-1 P. aeruginosa PU21 (pGIR-1)b P. aeruginosaPU21 E. coli DH10B (pDG-1)b E. coli DH10B Amoxicillin >512 >512 32 16 2 Ticarcillin 64 128 16 32 2 Ticarcillin plus CLA 64 128 16 32 1 Piperacillin 8 16 2 32 1 Piperacillin plus TZB 8 16 1 16 1 Cephalothin >128 >128 >128 8 4 Cefsulodin 256 64 2 32 2 Cefuroxime >128 >128 >128 4 4 Ceftazidime 256 512 0.25 128 0.06 Ceftazidime plus CLA 128 64 0.25 64 0.06 Cefotaxime 16 16 2 4 0.12 Cefepime 32 16 0.06 0.5 <0.06 Moxalactam 128 256 8 1 0.25 Aztreonam 32 32 0.5 8 0.12 Imipenem 2 0.25 0.12 0.06 0.06 - Table 2.
Amino acid differences among extended-spectrum oxacillinases compared to OXA-10
Oxacillinase Amino acid(s) at position(s)a: 10 20 28 58 70–73 76 92 110 127 144–146 164 167 184 208 216–218 240 258 272 OXA-10 I G S D S-T-F-K N V T A Y-G-N W G Y A K-T-G E S E OXA-M101b L OXA-M102b S D OXA-M103b K OXA-11 S D OXA-14 D OXA-16 T D OXA-17 S OXA-13 T S N S S F G N A OXA-13-1b T S N S S D F G N A OXA-19 T S N S D F G N A OXA-28 T S N S G F G N A
