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Mechanisms of Resistance

Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci

Jason Prystowsky, Farida Siddiqui, John Chosay, Dean L. Shinabarger, John Millichap, Lance R. Peterson, Gary A. Noskin
Jason Prystowsky
Department of Medicine, Division of Infectious Diseases, and
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Farida Siddiqui
Department of Medicine, Division of Infectious Diseases, and
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John Chosay
Pharmacia Corporation, Kalamazoo, Michigan
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Dean L. Shinabarger
Pharmacia Corporation, Kalamazoo, Michigan
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John Millichap
Department of Pathology, Clinical Microbiology Division, Northwestern University Medical School, and
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Lance R. Peterson
Department of Pathology, Clinical Microbiology Division, Northwestern University Medical School, and
the Prevention Epicenter, Northwestern Memorial Hospital, Chicago, Illinois, and
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Gary A. Noskin
Department of Medicine, Division of Infectious Diseases, and
the Prevention Epicenter, Northwestern Memorial Hospital, Chicago, Illinois, and
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DOI: 10.1128/AAC.45.7.2154-2156.2001
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ABSTRACT

To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, fiveEnterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E. faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 μg/ml contained a guanine to adenine transition at bp 2505.

  • Copyright © 2001 American Society for Microbiology
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Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci
Jason Prystowsky, Farida Siddiqui, John Chosay, Dean L. Shinabarger, John Millichap, Lance R. Peterson, Gary A. Noskin
Antimicrobial Agents and Chemotherapy Jul 2001, 45 (7) 2154-2156; DOI: 10.1128/AAC.45.7.2154-2156.2001

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Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci
Jason Prystowsky, Farida Siddiqui, John Chosay, Dean L. Shinabarger, John Millichap, Lance R. Peterson, Gary A. Noskin
Antimicrobial Agents and Chemotherapy Jul 2001, 45 (7) 2154-2156; DOI: 10.1128/AAC.45.7.2154-2156.2001
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KEYWORDS

Acetamides
Enterococcus
oxazolidinones
RNA, Ribosomal, 23S
vancomycin resistance

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