Article Figures & Data
Figures
- FIG. 1.
Activities of caspofungin at different concentrations against biofilms of C. albicans strain 3153A. Values are expressed as the average percentage of OD of wells containing treated biofilms compared to that of control wells (considered to be 100%) for the XTT assays. Results are from a single experiment performed with four replicate wells.
- FIG. 2.
Electron micrographs of preformed C. albicans 3153A biofilms on plastic coverslips after incubation for 24 h with medium alone (A) or with 0.5 μg of caspofungin per ml (B). Bar, 10 μm.
- FIG. 3.
CLSM of C. albicans 3153A biofilms on plastic coverslips after incubation for 24 h with medium alone (A) or with 0.5 μg of caspofungin per ml (B). Experiments utilize the FUN 1 stain to directly visualize the effects of caspofungin on preformed biofilms. Note the shift from green to red fluorescence visible in panel A (untreated control biofilms), which reflects processing of the dye by metabolically active cells. In contrast, caspofungin-treated biofilms (B) show diffuse green fluorescence characteristic of dead cells. Images are single xy optical sections taken across the z axis. Magnification, ×200.
- FIG. 4.
Effect of caspofungin impregnation on subsequent biofilm formation by C. albicans 3153A on the wells of microtiter plates. Values are expressed as the average percentage of OD (seven replicate wells) compared to that of control wells (considered to be 100%) for the XTT assays. Pretreatment at all caspofungin concentrations resulted in statistically significant differences in biofilm formation compared to that of control biofilms (P is 0.0379 for 0.03125 μg/ml and P is <0.001 for all other concentrations).
