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Mechanisms of Resistance

bla VIM-2 Cassette-Containing Novel Integrons in Metallo-β-Lactamase-Producing Pseudomonas aeruginosa and Pseudomonas putida Isolates Disseminated in a Korean Hospital

Kyungwon Lee, Jong Back Lim, Jong Hwa Yum, Dongeun Yong, Yunsop Chong, June Myung Kim, David M. Livermore
Kyungwon Lee
1Department of Clinical Pathology and Research Institute of Bacterial Resistance
3Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea
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Jong Back Lim
1Department of Clinical Pathology and Research Institute of Bacterial Resistance
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Jong Hwa Yum
1Department of Clinical Pathology and Research Institute of Bacterial Resistance
3Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea
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Dongeun Yong
1Department of Clinical Pathology and Research Institute of Bacterial Resistance
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Yunsop Chong
1Department of Clinical Pathology and Research Institute of Bacterial Resistance
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  • For correspondence: whonetkor@yumc.yonsei.ac.kr
June Myung Kim
2Department of Internal Medicine
3Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea
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David M. Livermore
4Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom
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DOI: 10.1128/AAC.46.4.1053-1058.2002
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  • FIG. 1.
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    FIG. 1.

    Schematic presentation of PFGE patterns of XbaI-digested genomic DNA of blaVIM-2-positive isolates of P. aeruginosa. M in lane 1 is a size marker (a chromosome of Saccharomyces cerevisiae). The numbers of isolates with serotypes O:11, O:12, and OPA are shown under each PFGE profile, and total numbers are in parentheses. OPA, O polyagglutination; NT, serotype not tested.

  • FIG. 2.
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    FIG. 2.

    Schematic structure (not to scale) of integrons in isolates of P. aeruginosa YMC 95/1/704 and P. putida YMC 97/8/322, which had approximate sizes of 5.6 and 3 kb, respectively. The ORFs are boxed. The names of unknown orfs “i,” “ii,” and “iii” are arbitrary. Arrows indicate their transcriptional orientation. orf “ii” had a reversed orientation. Primers used for sequencing are shown under the integron structures (see text for primer sequences).

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  • TABLE 1.

    Trend of isolation of imipenem-resistant Pseudomonas spp. and detection of blaVIM-2-positive isolates

    YrP. aeruginosaP. putida
    No. of isolates (% imipenem resistance)No. of blaVIM-2 isolatesNo. of isolates (% imipenem resistance)No. of blaVIM-2 isolates
    TestedPositive (%)TestedPositive (%)
    19941,939 (4)10 (0)38 (8)0NAa
    1995966 (3)205 (25.0)6 (0)0NA
    19962,189 (6)80 (0)37 (16)0NA
    19972,078 (16)13015 (11.5)17 (6)11 (100)
    19982,934 (15)14712 (8.2)35 (29)55 (100)
    19993,129 (15)18711 (5.9)35 (17)33 (100)
    Total13,235 (11)49343 (8.7)168 (15)99 (100)
    • ↵ a NA, not applicable.

  • TABLE 2.

    Antimicrobial susceptibilities of blaVIM-2-positive clinical isolates and their transconjugants

    Antimicrobial agentaP. aeruginosaP. putida
    CIb (n = 43)TCb (n = 6)CI (n = 9)TC (n = 2)
    MIC rangecMIC50cMIC90cMIC rangeMIC rangeMIC range
    Imipenem8->1281612816-3216-12816-32
    Meropenem8->128161288-3232-1288-16
    Piperacillin64->256256>2568-12864->256256
    Piperacillin-tazobactamd32->128128>1288-12832->256128
    Cefotaxime>128>128>12864->128>128>128
    Cefotaxime-clavulanic acidd>128>128>12832->128>128>128
    Ceftazidime32->128128>1288-6432->128128
    Aztreonam4-6416322-416-324
    Amikacin2->128>12816416->1284
    Gentamicin8->128>1281286432->12864
    Kanamycin64->128>128>128>12816->128>128
    Streptomycin128->128>128>128>128>128128
    Tobramycin2->128>1283216-3216->12832
    Ciprofloxacin0.12->12832160.12-0.25160.12
    • ↵ a MICs of ampicillin, cephalothin, and cefoxitin for all of the strains were >128 μg/ml.

    • ↵ b Abbreviations: CI, clinical isolates; TC, transconjugants.

    • ↵ c Values are in micrograms per milliliter; MIC50 and MIC90, MICs for 50 and 90% of strains, respectively.

    • ↵ d Piperacillin-tazobactam: tazobactam at a fixed concentration of 4 μg/ml; cefotaxime- clavulanic acid: a 2:1 combination.

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bla VIM-2 Cassette-Containing Novel Integrons in Metallo-β-Lactamase-Producing Pseudomonas aeruginosa and Pseudomonas putida Isolates Disseminated in a Korean Hospital
Kyungwon Lee, Jong Back Lim, Jong Hwa Yum, Dongeun Yong, Yunsop Chong, June Myung Kim, David M. Livermore
Antimicrobial Agents and Chemotherapy Apr 2002, 46 (4) 1053-1058; DOI: 10.1128/AAC.46.4.1053-1058.2002

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bla VIM-2 Cassette-Containing Novel Integrons in Metallo-β-Lactamase-Producing Pseudomonas aeruginosa and Pseudomonas putida Isolates Disseminated in a Korean Hospital
Kyungwon Lee, Jong Back Lim, Jong Hwa Yum, Dongeun Yong, Yunsop Chong, June Myung Kim, David M. Livermore
Antimicrobial Agents and Chemotherapy Apr 2002, 46 (4) 1053-1058; DOI: 10.1128/AAC.46.4.1053-1058.2002
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KEYWORDS

Integrins
Pseudomonas Infections
Pseudomonas aeruginosa
Pseudomonas putida
beta-lactamases

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