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Susceptibility

Effects of Chemical and Physical Agents on Viability and Infectivity of Encephalitozoon intestinalis Determined by Cell Culture and Flow Cytometry

Maud Santillana-Hayat, Claudine Sarfati, Sandra Fournier, Françoise Chau, Raphaël Porcher, Jean-Michel Molina, Francis Derouin
Maud Santillana-Hayat
1Parasitology-Mycology Laboratory
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Claudine Sarfati
1Parasitology-Mycology Laboratory
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Sandra Fournier
2Department of Infectious Diseases
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Françoise Chau
3INSERM, Bichat Hospital, Paris, France
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Raphaël Porcher
4Department of Biostatistics, Saint-Louis Hospital, AP-HP and EA 3520, University of Paris 7
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Jean-Michel Molina
2Department of Infectious Diseases
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Francis Derouin
1Parasitology-Mycology Laboratory
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  • For correspondence: paracord@wanadoo.fr
DOI: 10.1128/AAC.46.6.2049-2051.2002
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    FIG. 1.

    Assessment of the viability of spores of E. intestinalis by flow cytometry (fluorescence histograms). Spores were exposed to Surfanios for 5 min (a), 70% ethanol for 5 min (b), 0.1 N HCl for 5 min (c), or 0.1 N NaOH for 5 min (d).

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  • TABLE 1.

    Effect of a 5-min exposure to chemical agents and disinfectants on infectivity and viability of spores of E. intestinalis

    Treatment% of spores recoveredCulture titrationFlow cytometry
    Mean titera:Pc% Reduction% Viabilityb:% Reduction
    Before treatmentAfter treatmentBefore treatmentAfter treatment
    HCl, 0.1 N8490.5 (64-128)22.6 (16-32)0.000175795037
    NaOH, 0.1 N8684.4 (64-128)34.3 (32-64)0.00259795432
    Surfanios, 0.2%997.0 (64-256)0 (0-0)<10−4100802379
    Benzalkonium chlorideAggregated spores147.8 (128-256)NDdNDNDNDND
    Sodium hypochlorite (chlorine, 36 g/liter)Spores destroyed256 (256-256)NDNDNDNDND
    Ethanol, 70%43106.2 (64-256)0 (0-0)<10−4100782371
    • ↵ a Geometric mean (range) of the titer from 10 to 15 titrations.

    • ↵ b Percent nonfluorescent (viable) spores from a count of 10,000 spores.

    • ↵ c Wilcoxon rank-sum test.

    • ↵ d ND, not done.

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Effects of Chemical and Physical Agents on Viability and Infectivity of Encephalitozoon intestinalis Determined by Cell Culture and Flow Cytometry
Maud Santillana-Hayat, Claudine Sarfati, Sandra Fournier, Françoise Chau, Raphaël Porcher, Jean-Michel Molina, Francis Derouin
Antimicrobial Agents and Chemotherapy Jun 2002, 46 (6) 2049-2051; DOI: 10.1128/AAC.46.6.2049-2051.2002

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Effects of Chemical and Physical Agents on Viability and Infectivity of Encephalitozoon intestinalis Determined by Cell Culture and Flow Cytometry
Maud Santillana-Hayat, Claudine Sarfati, Sandra Fournier, Françoise Chau, Raphaël Porcher, Jean-Michel Molina, Francis Derouin
Antimicrobial Agents and Chemotherapy Jun 2002, 46 (6) 2049-2051; DOI: 10.1128/AAC.46.6.2049-2051.2002
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  • Top
  • Article
    • ABSTRACT
    • Effect of different temperatures.
    • Exposure to chemical and disinfectants.
    • Assessment of infectivity by tissue culture.
    • Assessment of viability by flow cytometry.
    • Statistical analysis.
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

disinfectants
Encephalitozoon

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