DOI: 10.1128/AAC.46.6.2049-2051.2002
Article Figures & Data
Figures
- FIG. 1.
Assessment of the viability of spores of E. intestinalis by flow cytometry (fluorescence histograms). Spores were exposed to Surfanios for 5 min (a), 70% ethanol for 5 min (b), 0.1 N HCl for 5 min (c), or 0.1 N NaOH for 5 min (d).
Tables
- TABLE 1.
Effect of a 5-min exposure to chemical agents and disinfectants on infectivity and viability of spores of E. intestinalis
Treatment % of spores recovered Culture titration Flow cytometry Mean titera: Pc % Reduction % Viabilityb: % Reduction Before treatment After treatment Before treatment After treatment HCl, 0.1 N 84 90.5 (64-128) 22.6 (16-32) 0.0001 75 79 50 37 NaOH, 0.1 N 86 84.4 (64-128) 34.3 (32-64) 0.002 59 79 54 32 Surfanios, 0.2% 9 97.0 (64-256) 0 (0-0) <10−4 100 80 23 79 Benzalkonium chloride Aggregated spores 147.8 (128-256) NDd ND ND ND ND Sodium hypochlorite (chlorine, 36 g/liter) Spores destroyed 256 (256-256) ND ND ND ND ND Ethanol, 70% 43 106.2 (64-256) 0 (0-0) <10−4 100 78 23 71
