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Mechanisms of Resistance

Novel Ciprofloxacin-Resistant, Nalidixic Acid-Susceptible Mutant of Staphylococcus aureus

Laura J. V. Piddock, Yu Fang Jin, Mark A. Webber, Martin J. Everett
Laura J. V. Piddock
Antimicrobial Agents Research Group, Division of Immunity & Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom
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  • For correspondence: L.j.v.piddock@bham.ac.uk
Yu Fang Jin
Antimicrobial Agents Research Group, Division of Immunity & Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom
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Mark A. Webber
Antimicrobial Agents Research Group, Division of Immunity & Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom
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Martin J. Everett
Antimicrobial Agents Research Group, Division of Immunity & Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom
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DOI: 10.1128/AAC.46.7.2276-2278.2002
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    FIG. 1.

    Accumulation of quinolones by F77 and F145. Data shown are mean values from three experiments; error bars indicate the standard deviation for each experiment. Abbreviations: Nal, nalidixic acid; Cip, ciprofloxacin; Nor, norfloxacin; CCCP, 100 μM CCCP. Symbols: □, F77; ▪, F145.

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  • TABLE 1.

    MICs of 15 quinolones and other antibiotics, dyes, detergents, and disinfectants for S. aureus F77 and F145

    Agent(s)aMICs (μg/ml) of S. aureus strains
    F77F145
    Antibiotics
        Nalidixic acid12816
        Nalidixic acid + reserpine0.250.25
        Nalidixic acid + CCCP0.250.25
        Ciprofloxacin0.254
        Ciprofloxacin + reserpine0.251.6
        Ciprofloxacin + CCCP0.250.25
        Norfloxacin216
        Norfloxacin + reserpine0.20.4
        Tetracyclineb0.50.3
    Dyes
        Acriflavine416
        Acridine orange84
        Ethidium bromide20.5
        Ethidium bromide + reserpine0.20.2
    Detergents
        Dehydrocholic acid256512
        Dehydrocholic acid + CCCP0.250.25
        Doxorubicin12.50.4
        CHAPS10241024
        SDS3216
        TX-1140.50.25
        NP-404<0.015
    Disinfectants
        Cetrimide0.254
    Efflux pump inhibitors
        TPP6432
        CCCP64512
        Reserpine128512
    • ↵ a CHAPS, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; SDS, sodium dodecyl sulfate; TX-114, Triton X-114; NP-40, Nonidet P-40; TPP, tetraphenylophosphonium.

    • ↵ b The MIC of tetracycline was determined three times using a decimal dilution method with increments of 0.1 μg/ml.

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Novel Ciprofloxacin-Resistant, Nalidixic Acid-Susceptible Mutant of Staphylococcus aureus
Laura J. V. Piddock, Yu Fang Jin, Mark A. Webber, Martin J. Everett
Antimicrobial Agents and Chemotherapy Jul 2002, 46 (7) 2276-2278; DOI: 10.1128/AAC.46.7.2276-2278.2002

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Novel Ciprofloxacin-Resistant, Nalidixic Acid-Susceptible Mutant of Staphylococcus aureus
Laura J. V. Piddock, Yu Fang Jin, Mark A. Webber, Martin J. Everett
Antimicrobial Agents and Chemotherapy Jul 2002, 46 (7) 2276-2278; DOI: 10.1128/AAC.46.7.2276-2278.2002
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KEYWORDS

anti-infective agents
ciprofloxacin
nalidixic acid
Staphylococcus aureus

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