Article Figures & Data
Figures
- FIG. 1.
Structure of the integron of pMAR-12. (A) In35 containing the blaCTX-M-2 gene obtained by sequencing pMAR-121 (2,100 bp) (region I) and PCR mapping of the surrounding regions (regions II and III); (B) lengths of PCR products; (C) sequence reported here (GenBank accession number AY079169).
- FIG. 2.
Comparison of the genetic structures of In6 (catII), In7(dfrA10), In35 (blaCTX-M-2), pSAL-1(blaDHA-1), and In60 (blaCTX-M-9). Sequences indicated by shaded boxes are identical. (A) A 28-bp segment at the end of the common region; (B) beginning of the common region of In6 and In7 (nucleotides in bold) that lies 24 bp beyond the stop codon of the sul1 gene (underlined) in the first 3′-CS; (C) open reading frame (orf) that is 71.6% identical to Orf3 from K. ascorbata (accession number AJ272538).
- FIG. 3.
Deletions at the 5′ end of the second 3′-CS in In60 (blaCTX-M-9), In35 (blaCTX-M-2), In7 (dfrA10), pSAL-1 (blaDHA-1), and In6 (catII). Base 1 corresponds to the first base of the 3′-CS.
Tables
- TABLE 1.
Oligonucleotides used for PCR mapping
Primer Primer sequence (5′ to 3′) Region Accession no. 1 GGC ATC CAA GCA GCA AG 5′-CS M73819 2 AAG CAG ACT TGA CCT GA 3′-CS M73819 3 CAT TCT GCG GTC GGC TT OrfD gene X72585 4 AAT AGC AGG GGT AGC GT blaCTX-M-2 gene X92507 5 GCG AAG TAA TCG CAA CAT CC 3′-CS U49101 6 TCA CTT TAT CGG GAC CAC blaCTX-M-2 gene X92507 7 GAC GGT GTT CGG CAT TCT 3′-CS U49101 8 CGT CGG GAT ATT CTG GC blaCTX-M-2 gene X92507 9 AGC CCC ATA CCT ACA AAG CC 3′-CS U49101 10 TTT GAA GGT TCG ACA GC 3′-CS U49101 11 AAA CAC GCC AGG CAT TC aac(6′)-Ib gene AF231133 12 CGC AGA TCA GTT GGA AG aadA gene AF326210 13 CCG CAG CTA GAA TTT TG aadB gene X04555
