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Mechanisms of Resistance

bla CTX-M-2 Is Located in an Unusual Class 1 Integron (In35) Which Includes Orf513

Sonia M. Arduino, Paul H. Roy, George A. Jacoby, Betina E. Orman, Silvia A. Pineiro, Daniela Centron
Sonia M. Arduino
1Facultad de Medicina de la Universidad de Buenos Aires, Paraguay 2155 (1121)Capital Federal, Argentina
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  • For correspondence: sarduino@aol.com
Paul H. Roy
2Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Sainte-Foy, Québec G1K 7P4, and Centre de Recherche en Infectiologie, Centre de Recherche du CHUL, Sainte-Foy, Québec G1V 4G2, Canada
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George A. Jacoby
3Edith Nourse Rogers Memorial Veterans Hospital, Bedford, and Lahey Clinic, Burlington, Massachusetts
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Betina E. Orman
1Facultad de Medicina de la Universidad de Buenos Aires, Paraguay 2155 (1121)Capital Federal, Argentina
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Silvia A. Pineiro
1Facultad de Medicina de la Universidad de Buenos Aires, Paraguay 2155 (1121)Capital Federal, Argentina
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Daniela Centron
1Facultad de Medicina de la Universidad de Buenos Aires, Paraguay 2155 (1121)Capital Federal, Argentina
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DOI: 10.1128/AAC.46.7.2303-2306.2002
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    FIG. 1.

    Structure of the integron of pMAR-12. (A) In35 containing the blaCTX-M-2 gene obtained by sequencing pMAR-121 (2,100 bp) (region I) and PCR mapping of the surrounding regions (regions II and III); (B) lengths of PCR products; (C) sequence reported here (GenBank accession number AY079169 ).

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    FIG. 2.

    Comparison of the genetic structures of In6 (catII), In7(dfrA10), In35 (blaCTX-M-2), pSAL-1(blaDHA-1), and In60 (blaCTX-M-9). Sequences indicated by shaded boxes are identical. (A) A 28-bp segment at the end of the common region; (B) beginning of the common region of In6 and In7 (nucleotides in bold) that lies 24 bp beyond the stop codon of the sul1 gene (underlined) in the first 3′-CS; (C) open reading frame (orf) that is 71.6% identical to Orf3 from K. ascorbata (accession number AJ272538 ).

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    FIG. 3.

    Deletions at the 5′ end of the second 3′-CS in In60 (blaCTX-M-9), In35 (blaCTX-M-2), In7 (dfrA10), pSAL-1 (blaDHA-1), and In6 (catII). Base 1 corresponds to the first base of the 3′-CS.

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  • TABLE 1.

    Oligonucleotides used for PCR mapping

    PrimerPrimer sequence (5′ to 3′)RegionAccession no.
    1 GGC ATC CAA GCA GCA AG 5′-CS M73819
    2 AAG CAG ACT TGA CCT GA 3′-CS M73819
    3 CAT TCT GCG GTC GGC TT OrfD gene X72585
    4 AAT AGC AGG GGT AGC GT bla CTX-M-2 gene X92507
    5 GCG AAG TAA TCG CAA CAT CC 3′-CS U49101
    6 TCA CTT TAT CGG GAC CAC bla CTX-M-2 gene X92507
    7 GAC GGT GTT CGG CAT TCT 3′-CS U49101
    8 CGT CGG GAT ATT CTG GC bla CTX-M-2 gene X92507
    9 AGC CCC ATA CCT ACA AAG CC 3′-CS U49101
    10 TTT GAA GGT TCG ACA GC 3′-CS U49101
    11 AAA CAC GCC AGG CAT TC aac(6′)-Ib gene AF231133
    12 CGC AGA TCA GTT GGA AG aadA gene AF326210
    13 CCG CAG CTA GAA TTT TG aadB gene X04555
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bla CTX-M-2 Is Located in an Unusual Class 1 Integron (In35) Which Includes Orf513
Sonia M. Arduino, Paul H. Roy, George A. Jacoby, Betina E. Orman, Silvia A. Pineiro, Daniela Centron
Antimicrobial Agents and Chemotherapy Jul 2002, 46 (7) 2303-2306; DOI: 10.1128/AAC.46.7.2303-2306.2002

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bla CTX-M-2 Is Located in an Unusual Class 1 Integron (In35) Which Includes Orf513
Sonia M. Arduino, Paul H. Roy, George A. Jacoby, Betina E. Orman, Silvia A. Pineiro, Daniela Centron
Antimicrobial Agents and Chemotherapy Jul 2002, 46 (7) 2303-2306; DOI: 10.1128/AAC.46.7.2303-2306.2002
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KEYWORDS

Genes, Bacterial
beta-lactamases

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