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Mechanisms of Resistance

Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis

Juan-Carlos Palomino, Anandi Martin, Mirtha Camacho, Humberto Guerra, Jean Swings, Françoise Portaels
Juan-Carlos Palomino
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000
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  • For correspondence: palomino@itg.be
Anandi Martin
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000
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Mirtha Camacho
2Tuberculosis Laboratory, Instituto Nacional de Laboratorios de Salud, La Paz, Bolivia
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Humberto Guerra
3Instituto de Medicina Tropical “Alexander von Humboldt,” Universidad Peruana Cayetano Heredia, Lima 100, Peru
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Jean Swings
4Laboratorium voor Microbiologie
5BCCM/LMG Culture Collection, Universiteit Gent, B-9000 Ghent, Belgium
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Françoise Portaels
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000
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DOI: 10.1128/AAC.46.8.2720-2722.2002
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Tables

  • TABLE 1.

    MICs of INH and RIF for 80 M. tuberculosis isolates determined by using the REMA plate method

    Result with PM (n)No. of isolates for which INH MIC (μg/ml) was:No. of isolates for which RIF MIC (μg/ml) was:
    ≤0.030.060.250.51.0>1.0≤0.060.1250.250.5>2.0
    INH resistant (54)361332
    INH susceptible (26)1871
    RIF resistant (49)148
    RIF susceptible (31)14710
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Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis
Juan-Carlos Palomino, Anandi Martin, Mirtha Camacho, Humberto Guerra, Jean Swings, Françoise Portaels
Antimicrobial Agents and Chemotherapy Aug 2002, 46 (8) 2720-2722; DOI: 10.1128/AAC.46.8.2720-2722.2002

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Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis
Juan-Carlos Palomino, Anandi Martin, Mirtha Camacho, Humberto Guerra, Jean Swings, Françoise Portaels
Antimicrobial Agents and Chemotherapy Aug 2002, 46 (8) 2720-2722; DOI: 10.1128/AAC.46.8.2720-2722.2002
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KEYWORDS

antitubercular agents
microbial sensitivity tests
Mycobacterium tuberculosis
Oxazines
Xanthenes

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