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Susceptibility

Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method

Anandi Martin, Mirtha Camacho, Françoise Portaels, Juan Carlos Palomino
Anandi Martin
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium
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  • For correspondence: amartin@itg.be
Mirtha Camacho
2Tuberculosis Laboratory, Instituto Nacional de Laboratorios de Salud, Miraflores, La Paz, Bolivia
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Françoise Portaels
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium
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Juan Carlos Palomino
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium
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DOI: 10.1128/AAC.47.11.3616-3619.2003
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ABSTRACT

The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries.

  • Copyright © 2003 American Society for Microbiology
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Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method
Anandi Martin, Mirtha Camacho, Françoise Portaels, Juan Carlos Palomino
Antimicrobial Agents and Chemotherapy Oct 2003, 47 (11) 3616-3619; DOI: 10.1128/AAC.47.11.3616-3619.2003

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Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method
Anandi Martin, Mirtha Camacho, Françoise Portaels, Juan Carlos Palomino
Antimicrobial Agents and Chemotherapy Oct 2003, 47 (11) 3616-3619; DOI: 10.1128/AAC.47.11.3616-3619.2003
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KEYWORDS

antitubercular agents
microbial sensitivity tests
Mycobacterium tuberculosis
Oxazines
Xanthenes

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