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Mechanisms of Resistance

Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania

Fred C. Tenover, Linda M. Weigel, Peter C. Appelbaum, Linda K. McDougal, Jasmine Chaitram, Sigrid McAllister, Nancye Clark, George Killgore, Caroline M. O'Hara, Laura Jevitt, Jean B. Patel, Bülent Bozdogan
Fred C. Tenover
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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  • For correspondence: fnt1@cdc.gov
Linda M. Weigel
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Peter C. Appelbaum
2Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania 17033
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Linda K. McDougal
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Jasmine Chaitram
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Sigrid McAllister
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Nancye Clark
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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George Killgore
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Caroline M. O'Hara
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Laura Jevitt
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Jean B. Patel
1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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Bülent Bozdogan
2Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania 17033
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DOI: 10.1128/AAC.48.1.275-280.2004
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ABSTRACT

A vancomycin-resistant Staphylococcus aureus (VRSA) isolate was obtained from a patient in Pennsylvania in September 2002. Species identification was confirmed by standard biochemical tests and analysis of 16S ribosomal DNA, gyrA, and gyrB sequences; all of the results were consistent with the S. aureus identification. The MICs of a variety of antimicrobial agents were determined by broth microdilution and macrodilution methods following National Committee for Clinical Laboratory Standards (NCCLS) guidelines. The isolate was resistant to vancomycin (MIC = 32 μg/ml), aminoglycosides, β-lactams, fluoroquinolones, macrolides, and tetracycline, but it was susceptible to linezolid, minocycline, quinupristin-dalfopristin, rifampin, teicoplanin, and trimethoprim-sulfamethoxazole. The isolate, which was originally detected by using disk diffusion and a vancomycin agar screen plate, was vancomycin susceptible by automated susceptibility testing methods. Pulsed-field gel electrophoresis (PFGE) of SmaI-digested genomic DNA indicated that the isolate belonged to the USA100 lineage (also known as the New York/Japan clone), the most common staphylococcal PFGE type found in hospitals in the United States. The VRSA isolate contained two plasmids of 120 and 4 kb and was positive for mecA and vanA by PCR amplification. The vanA sequence was identical to the vanA sequence present in Tn1546. A DNA probe for vanA hybridized to the 120-kb plasmid. This is the second VRSA isolate reported in the United States.

  • Copyright © 2004 American Society for Microbiology
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Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania
Fred C. Tenover, Linda M. Weigel, Peter C. Appelbaum, Linda K. McDougal, Jasmine Chaitram, Sigrid McAllister, Nancye Clark, George Killgore, Caroline M. O'Hara, Laura Jevitt, Jean B. Patel, Bülent Bozdogan
Antimicrobial Agents and Chemotherapy Dec 2003, 48 (1) 275-280; DOI: 10.1128/AAC.48.1.275-280.2004

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Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania
Fred C. Tenover, Linda M. Weigel, Peter C. Appelbaum, Linda K. McDougal, Jasmine Chaitram, Sigrid McAllister, Nancye Clark, George Killgore, Caroline M. O'Hara, Laura Jevitt, Jean B. Patel, Bülent Bozdogan
Antimicrobial Agents and Chemotherapy Dec 2003, 48 (1) 275-280; DOI: 10.1128/AAC.48.1.275-280.2004
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KEYWORDS

Anti-Bacterial Agents
staphylococcal infections
vancomycin
vancomycin resistance

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